Toward a Physical Map of Drosophila Buzzatii. Use of Randomly Amplified Polymorphic Dna Polymorphisms and Sequence-tagged Site Landmarks

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 2000 Dec 5

PMID 11102375

Citations 9

Authors

H Laayouni

M Santos

A Fontdevila

Affiliations

Soon will be listed here.

Abstract

We present a physical map based on RAPD polymorphic fragments and sequence-tagged sites (STSs) for the repleta group species Drosophila buzzatii. One hundred forty-four RAPD markers have been used as probes for in situ hybridization to the polytene chromosomes, and positive results allowing the precise localization of 108 RAPDs were obtained. Of these, 73 behave as effectively unique markers for physical map construction, and in 9 additional cases the probes gave two hybridization signals, each on a different chromosome. Most markers (68%) are located on chromosomes 2 and 4, which partially agree with previous estimates on the distribution of genetic variation over chromosomes. One RAPD maps close to the proximal breakpoint of inversion 2z(3) but is not included within the inverted fragment. However, it was possible to conclude from this RAPD that the distal breakpoint of 2z(3) had previously been wrongly assigned. A total of 39 cytologically mapped RAPDs were converted to STSs and yielded an aggregate sequence of 28,431 bp. Thirty-six RAPDs (25%) did not produce any detectable hybridization signal, and we obtained the DNA sequence from three of them. Further prospects toward obtaining a more developed genetic map than the one currently available for D. buzzatii are discussed.

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References

Caceres M, Ranz J, Barbadilla A, Long M, Ruiz A . Generation of a widespread Drosophila inversion by a transposable element. Science. 1999; 285(5426):415-8. DOI: 10.1126/science.285.5426.415. View

Zouros E . The distribution of enzyme and inversion polymorphism over the genome of Drosophila: evidence against balancing selection. Genetics. 1976; 83(1):169-79. PMC: 1213498. DOI: 10.1093/genetics/83.1.169. View

Laird C . DNA of Drosophila chromosomes. Annu Rev Genet. 1973; 7:177-204. DOI: 10.1146/annurev.ge.07.120173.001141. View

Halliburton R, Barker J . Lack of mitochondrial DNA variation in Australian Drosophila buzzatii. Mol Biol Evol. 1993; 10(2):484-7. DOI: 10.1093/oxfordjournals.molbev.a040014. View

Gall J, COHEN E, Polan M . Reptitive DNA sequences in drosophila. Chromosoma. 1971; 33(3):319-44. DOI: 10.1007/BF00284948. View

Leibowitz A, Santos M, Fontdevila A . Heritability and selection on body size in a natural population of Drosophila buzzatii. Genetics. 1995; 141(1):181-9. PMC: 1206716. DOI: 10.1093/genetics/141.1.181. View

Smith J, Leadbetter J, Bush G, Roberts D, Fulbright D . Characterization of random amplified polymorphic DNA (RAPD) products from Xanthomonas campestris and some comments on the use of RAPD products in phylogenetic analysis. Mol Phylogenet Evol. 1994; 3(2):135-45. DOI: 10.1006/mpev.1994.1016. View

Apostol B, Black 4th W, Reiter P, Miller B . Population genetics with RAPD-PCR markers: the breeding structure of Aedes aegypti in Puerto Rico. Heredity (Edinb). 1996; 76 ( Pt 4):325-34. DOI: 10.1038/hdy.1996.50. View

Ruiz A, Wasserman M . Evolutionary cytogenetics of the Drosophila buzzatii species complex. Heredity (Edinb). 1993; 70 ( Pt 6):582-96. DOI: 10.1038/hdy.1993.85. View

10.

Naveira H, Fontdevila A . The Evolutionary History of DROSOPHILA BUZZATII. Xii. the Genetic Basis of Sterility in Hybrids between D. BUZZATII and Its Sibling D. SERIDO from Argentina. Genetics. 1986; 114(3):841-57. PMC: 1203016. DOI: 10.1093/genetics/114.3.841. View

11.

Olson M, Hood L, Cantor C, Botstein D . A common language for physical mapping of the human genome. Science. 1989; 245(4925):1434-5. DOI: 10.1126/science.2781285. View

12.

Louis C, Madueno E, Modolell J, Omar M, Papagiannakis G, Saunders R . One-hundred and five new potential Drosophila melanogaster genes revealed through STS analysis. Gene. 1997; 195(2):187-93. DOI: 10.1016/s0378-1119(97)00138-8. View

13.

Santos M, Ruiz A, Fontdevila A . The evolutionary history of Drosophila buzzatii. XXV. Random mating in nature. Heredity (Edinb). 1992; 68 ( Pt 4):373-9. DOI: 10.1038/hdy.1992.53. View

14.

Labrador M, Naveira H, Fontdevila A . Genetic mapping of the Adh locus in the repleta group of Drosophila by in situ hybridization. J Hered. 1990; 81(1):83-6. DOI: 10.1093/oxfordjournals.jhered.a110934. View

15.

Hartl D, Nurminsky D, Jones R, Lozovskaya E . Genome structure and evolution in Drosophila: applications of the framework P1 map. Proc Natl Acad Sci U S A. 1994; 91(15):6824-9. PMC: 44290. DOI: 10.1073/pnas.91.15.6824. View

16.

Ranz J, Segarra C, Ruiz A . Chromosomal homology and molecular organization of Muller's elements D and E in the Drosophila repleta species group. Genetics. 1997; 145(2):281-95. PMC: 1207795. DOI: 10.1093/genetics/145.2.281. View

17.

Dimopoulos G, Zheng L, Kumar V, Della Torre A, Kafatos F, Louis C . Integrated genetic map of Anopheles gambiae: use of RAPD polymorphisms for genetic, cytogenetic and STS landmarks. Genetics. 1996; 143(2):953-60. PMC: 1207351. DOI: 10.1093/genetics/143.2.953. View

18.

von Allmen G, Hogga I, Spierer A, Karch F, Bender W, Gyurkovics H . Splits in fruitfly Hox gene complexes. Nature. 1996; 380(6570):116. DOI: 10.1038/380116a0. View

19.

G Williams J, Kubelik A, Livak K, Rafalski J, Tingey S . DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990; 18(22):6531-5. PMC: 332606. DOI: 10.1093/nar/18.22.6531. View

20.

Naveira H, Fontdevila A . The evolutionary history of D. buzzatii. XXII. Chromosomal and genic sterility in male hybrids of Drosophila buzzatii and Drosophila koepferae. Heredity (Edinb). 1991; 66 ( Pt 2):233-9. DOI: 10.1038/hdy.1991.29. View