DNA Damage in the Nucleosome Core is Refractory to Repair by Human Excision Nuclease

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 2000 Nov 30

PMID 11094069

Citations 89

Authors

R Hara

J Mo

A Sancar

Affiliations

Soon will be listed here.

Abstract

To investigate the effect of nucleosomes on nucleotide excision repair in humans, we prepared a mononucleosome containing a (6-4) photoproduct in the nucleosome core and examined its repair with the reconstituted human excision nuclease system and with cell extracts. Nucleosomal DNA is repaired at a rate of about 10% of that for naked DNA in both systems. These results are in agreement with in vivo data showing a considerably slower rate of repair of overall genomic DNA relative to that for transcriptionally active DNA. Furthermore, our results indicate that the first-order packing of DNA in nucleosomes is a primary determinant of slow repair of DNA in chromatin.

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