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Constitutive Expression of the Neuron-restrictive Silencer Factor (NRSF)/REST in Differentiating Neurons Disrupts Neuronal Gene Expression and Causes Axon Pathfinding Errors in Vivo

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Specialty Science
Date 2000 Oct 26
PMID 11050251
Citations 57
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Abstract

The neuron-restrictive silencer factor (NRSF; also known as REST for repressor element-1 silencing transcription factor) is a transcriptional repressor of multiple neuronal genes, but little is known about its function in vivo. NRSF is normally down-regulated upon neuronal differentiation. Constitutive expression of NRSF in the developing spinal cord of chicken embryos caused repression of two endogenous target genes, N-tubulin and Ng-CAM, but did not prevent overt neurogenesis. Nevertheless, commissural neurons that differentiated while constitutively expressing NRSF showed a significantly increased frequency of axon guidance errors. These data suggest that down-regulation of NRSF is necessary for the proper development of at least some classes of neurons in vivo.

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References
1.
Tomita K, Ishibashi M, Nakahara K, Ang S, Nakanishi S, Guillemot F . Mammalian hairy and Enhancer of split homolog 1 regulates differentiation of retinal neurons and is essential for eye morphogenesis. Neuron. 1996; 16(4):723-34. DOI: 10.1016/s0896-6273(00)80093-8. View

2.
BESSIS A, Salmon A, Zoli M, Le Novere N, Picciotto M, Changeux J . Promoter elements conferring neuron-specific expression of the beta 2-subunit of the neuronal nicotinic acetylcholine receptor studied in vitro and in transgenic mice. Neuroscience. 1995; 69(3):807-19. DOI: 10.1016/0306-4522(95)00303-z. View

3.
Wood I, Roopra A, Buckley N . Neural specific expression of the m4 muscarinic acetylcholine receptor gene is mediated by a RE1/NRSE-type silencing element. J Biol Chem. 1996; 271(24):14221-5. DOI: 10.1074/jbc.271.24.14221. View

4.
Morgan B, Fekete D . Manipulating gene expression with replication-competent retroviruses. Methods Cell Biol. 1996; 51:185-218. DOI: 10.1016/s0091-679x(08)60629-9. View

5.
Schoenherr C, Paquette A, Anderson D . Identification of potential target genes for the neuron-restrictive silencer factor. Proc Natl Acad Sci U S A. 1996; 93(18):9881-6. PMC: 38523. DOI: 10.1073/pnas.93.18.9881. View