Role of TnrA in Nitrogen Source-dependent Repression of Bacillus Subtilis Glutamate Synthase Gene Expression

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2000 Oct 13

PMID 11029411

Citations 36

Authors

B R Belitsky

L V Wray Jr

S H FISHER

D E Bohannon

A L Sonenshein

Affiliations

Soon will be listed here.

Abstract

Synthesis of glutamate, the cell's major donor of nitrogen groups and principal anion, occupies a significant fraction of bacterial metabolism. In Bacillus subtilis, the gltAB operon, encoding glutamate synthase, requires a specific positive regulator, GltC, for its expression. In addition, the gltAB operon was shown to be repressed by TnrA, a regulator of several other genes of nitrogen metabolism and active under conditions of ammonium (nitrogen) limitation. TnrA was found to bind directly to a site immediately downstream of the gltAB promoter. As is true for other genes, the activity of TnrA at the gltAB promoter was antagonized by glutamine synthetase under certain growth conditions.

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