Crystallization of Antimicrobial Pores in Membranes: Magainin and Protegrin

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2000 Oct 12

PMID 11023904

Citations 138

Authors

L Yang

T M Weiss

R I Lehrer

H W Huang

Affiliations

Soon will be listed here.

Abstract

Membrane pores spontaneously formed by antimicrobial peptides in membranes were crystallized for the first time by manipulating the sample hydration and temperature. Neutron diffraction shows that magainins and protegrins form stable pores in fully hydrated fluid membranes. At lower hydration levels or low temperature, the membrane multilayers crystallize. In one crystalline phase, the pores in each bilayer arrange in a regular hexagonal array and the bilayers are stacked into a hexagonal ABC lattice, corresponding to the cubic close-packed structure of spheres. In another crystalline phase, the bilayers are modulated into the rippled multilamellae, corresponding to a 2D monoclinic lattice. The phase diagrams are described. Crystallization of the membrane pores provides possibilities for diffraction studies that might provide useful information on the pore structures.

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References

Bessalle R, Kapitkovsky A, Gorea A, Shalit I, Fridkin M . All-D-magainin: chirality, antimicrobial activity and proteolytic resistance. FEBS Lett. 1990; 274(1-2):151-5. DOI: 10.1016/0014-5793(90)81351-n. View

Tamamura H, Murakami T, Horiuchi S, Sugihara K, Otaka A, TAKADA W . Synthesis of protegrin-related peptides and their antibacterial and anti-human immunodeficiency virus activity. Chem Pharm Bull (Tokyo). 1995; 43(5):853-8. DOI: 10.1248/cpb.43.853. View

He K, Ludtke S, Heller W, Huang H . Mechanism of alamethicin insertion into lipid bilayers. Biophys J. 1996; 71(5):2669-79. PMC: 1233753. DOI: 10.1016/S0006-3495(96)79458-4. View

Heller W, Waring A, Lehrer R, Harroun T, Weiss T, Yang L . Membrane thinning effect of the beta-sheet antimicrobial protegrin. Biochemistry. 2000; 39(1):139-45. DOI: 10.1021/bi991892m. View

Wack , Webb . Synchrotron x-ray study of the modulated lamellar phase P beta ' in the lecithin-water system. Phys Rev A Gen Phys. 1989; 40(5):2712-2730. DOI: 10.1103/physreva.40.2712. View

Yang L, Harroun T, Heller W, Weiss T, Huang H . Neutron off-plane scattering of aligned membranes. I. Method Of measurement. Biophys J. 1998; 75(2):641-5. PMC: 1299739. DOI: 10.1016/S0006-3495(98)77554-X. View

Christensen B, Fink J, MERRIFIELD R, Mauzerall D . Channel-forming properties of cecropins and related model compounds incorporated into planar lipid membranes. Proc Natl Acad Sci U S A. 1988; 85(14):5072-6. PMC: 281690. DOI: 10.1073/pnas.85.14.5072. View

Ludtke S, He K, Wu Y, Huang H . Cooperative membrane insertion of magainin correlated with its cytolytic activity. Biochim Biophys Acta. 1994; 1190(1):181-4. DOI: 10.1016/0005-2736(94)90050-7. View

Wade D, Boman A, Wahlin B, Drain C, Andreu D, Boman H . All-D amino acid-containing channel-forming antibiotic peptides. Proc Natl Acad Sci U S A. 1990; 87(12):4761-5. PMC: 54197. DOI: 10.1073/pnas.87.12.4761. View

10.

Zasloff M . Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of a precursor. Proc Natl Acad Sci U S A. 1987; 84(15):5449-53. PMC: 298875. DOI: 10.1073/pnas.84.15.5449. View

11.

Wu Y, Huang H, Olah G . Method of oriented circular dichroism. Biophys J. 1990; 57(4):797-806. PMC: 1280780. DOI: 10.1016/S0006-3495(90)82599-6. View

12.

Cruciani R, Barker J, Zasloff M, Chen H, Colamonici O . Antibiotic magainins exert cytolytic activity against transformed cell lines through channel formation. Proc Natl Acad Sci U S A. 1991; 88(9):3792-6. PMC: 51539. DOI: 10.1073/pnas.88.9.3792. View

13.

Huang H . Action of antimicrobial peptides: two-state model. Biochemistry. 2000; 39(29):8347-52. DOI: 10.1021/bi000946l. View

14.

Kokryakov V, Harwig S, Panyutich E, Shevchenko A, Aleshina G, Shamova O . Protegrins: leukocyte antimicrobial peptides that combine features of corticostatic defensins and tachyplesins. FEBS Lett. 1993; 327(2):231-6. DOI: 10.1016/0014-5793(93)80175-t. View

15.

Wu Y, He K, Ludtke S, Huang H . X-ray diffraction study of lipid bilayer membranes interacting with amphiphilic helical peptides: diphytanoyl phosphatidylcholine with alamethicin at low concentrations. Biophys J. 1995; 68(6):2361-9. PMC: 1282146. DOI: 10.1016/S0006-3495(95)80418-2. View

16.

Ludtke S, He K, Heller W, Harroun T, Yang L, Huang H . Membrane pores induced by magainin. Biochemistry. 1996; 35(43):13723-8. DOI: 10.1021/bi9620621. View

17.

He K, Ludtke S, Worcester D, Huang H . Neutron scattering in the plane of membranes: structure of alamethicin pores. Biophys J. 1996; 70(6):2659-66. PMC: 1225245. DOI: 10.1016/S0006-3495(96)79835-1. View

18.

Blaurock A . Structure of the nerve myelin membrane: proof of the low-resolution profile. J Mol Biol. 1971; 56(1):35-52. DOI: 10.1016/0022-2836(71)90082-9. View

19.

Aumelas A, Mangoni M, Roumestand C, Chiche L, Despaux E, Grassy G . Synthesis and solution structure of the antimicrobial peptide protegrin-1. Eur J Biochem. 1996; 237(3):575-83. DOI: 10.1111/j.1432-1033.1996.0575p.x. View

20.

Hoffmann J, Kafatos F, Janeway C, Ezekowitz R . Phylogenetic perspectives in innate immunity. Science. 1999; 284(5418):1313-8. DOI: 10.1126/science.284.5418.1313. View