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Multiple Steps in DNA Recognition by Restriction Endonuclease from E. Coli K

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Journal Nature
Specialty Science
Date 1975 Aug 14
PMID 1101070
Citations 21
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Abstract

The process of DNA recognition by the activated form of the restriction endonuclease from E. coli K involves three enzyme-DNA complexes which can be differentiated experimentally. These are: an initial complex formed at a nonspecific site; a recognition complex involving the host specificity site; and a cleavage complex dependent on the presence of ATP.

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