Sequence of 1000 Nucleotides at the 3' End of Tobacco Mosaic Virus RNA

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1979 Apr 1

PMID 109810

Citations 43

Authors

H Guilley

G Jonard

B Kukla

K E Richards

Affiliations

Soon will be listed here.

Abstract

The sequence of 1000 nucleotides at the 3' end of tobacco mosaic virus RNA has been determined. The sequence contains the entire coat protein cistron as well as regions to its left and right. Sequence characterization was by conventional methods for use with uniformly 32P labeled RNA complemented by newer methods for in vitro 5' and 3' 32P end-labeling of RNA and its subsequent rapid analysis. The noncoding region separating the coat protein cistron from the 3' terminus is 204 residues long and may be folded into a clover-leaf-type secondary structure. The distribution of termination codons to the left of the coat protein cistron suggests that the end of the adjacent cistron is separated from the beginning of the coat protein cistron by only two nucleotides. The subgenomic viral coat protein mRNA was isolated from infected tissue and shown to be capped. The nontranslated sequence separating the cap from the AUG initiation codon is 9 residues long and thus overlaps a portion of the adjacent cistron on the genome RNA.

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References

Shinshi H, Miwa M, Kato K, Noguchi M, Matsushima T, Sugimura T . A novel phosphodiesterase from cultured tobacco cells. Biochemistry. 1976; 15(10):2185-90. DOI: 10.1021/bi00655a024. View

Perham R, Wilson T . The characterization of intermediates formed during the disassembly of tobacco mosaic virus at alkaline pH. Virology. 1978; 84(2):293-302. DOI: 10.1016/0042-6822(78)90249-0. View

Lockard R, Heckman J, MACGEE J, Tabor M, RajBhandary U . Sequence analysis of 5'[32P] labeled mRNA and tRNA using polyacrylamide gel electrophoresis. Nucleic Acids Res. 1978; 5(1):37-56. PMC: 341960. DOI: 10.1093/nar/5.1.37. View

Zimmern D . The 5' end group of tobacco mosaic virus RNA is m7G5' ppp5' Gp. Nucleic Acids Res. 1975; 2(7):1189-201. PMC: 343505. DOI: 10.1093/nar/2.7.1189. View

Silberklang M, Gillum A, RajBhandary U . The use of nuclease P1 in sequence analysis of end group labeled RNA. Nucleic Acids Res. 1977; 4(12):4091-108. PMC: 343228. DOI: 10.1093/nar/4.12.4091. View

Efstratiadis A, Vournakis J, Donis-Keller H, Chaconas G, Dougall D, Kafatos F . End labeling of enzymatically decapped mRNA. Nucleic Acids Res. 1977; 4(12):4165-74. PMC: 343232. DOI: 10.1093/nar/4.12.4165. View

Maxam A, Gilbert W . A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977; 74(2):560-4. PMC: 392330. DOI: 10.1073/pnas.74.2.560. View

Dasgupta R, Kaesberg P . Sequence of an oligonucleotide derived from the 3' end of each of the four brome mosaic viral RNAs. Proc Natl Acad Sci U S A. 1977; 74(11):4900-4. PMC: 432064. DOI: 10.1073/pnas.74.11.4900. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

10.

Lockard R, RajBhandary U, Bol J . Nucleotide sequence of 5' terminus of alfalfa mosaic virus RNA 4 leading into coat protein cistron. Proc Natl Acad Sci U S A. 1977; 74(12):5504-8. PMC: 431782. DOI: 10.1073/pnas.74.12.5504. View

11.

Silberklang M, Prochiantz A, Haenni A, RajBhandary U . Studies on the sequence of the 3'-terminal region of turnip-yellow-mosaic-virus RNA. Eur J Biochem. 1977; 72(3):465-78. DOI: 10.1111/j.1432-1033.1977.tb11270.x. View

12.

Donis-Keller H, Maxam A, Gilbert W . Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977; 4(8):2527-38. PMC: 342589. DOI: 10.1093/nar/4.8.2527. View

13.

SANGER F, Coulson A . The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978; 87(1):107-10. DOI: 10.1016/0014-5793(78)80145-8. View

14.

Pelham H . Leaky UAG termination codon in tobacco mosaic virus RNA. Nature. 1978; 272(5652):469-71. DOI: 10.1038/272469a0. View

15.

Richards K, Guilley H, Jonard G, Hirth L . Nucleotide sequence at the 5' extremity of tobacco-mosaic-virus RNA. 1. The noncoding region (nucleotides 1-68). Eur J Biochem. 1978; 84(2):513-9. DOI: 10.1111/j.1432-1033.1978.tb12194.x. View

16.

Gross H, Domdey H, Lossow C, Jank P, Raba M, Alberty H . Nucleotide sequence and secondary structure of potato spindle tuber viroid. Nature. 1978; 273(5659):203-8. DOI: 10.1038/273203a0. View

17.

England T, Uhlenbeck O . 3'-terminal labelling of RNA with T4 RNA ligase. Nature. 1978; 275(5680):560-1. DOI: 10.1038/275560a0. View

18.

Guilley H, Briand J . Nucleotide sequence of turnip yellow mosaic virus coat protein mRNA. Cell. 1978; 15(1):113-22. DOI: 10.1016/0092-8674(78)90087-9. View

19.

Dasgupta R, Shih D, Saris C, Kaesberg P . Nucleotide sequence of a viral RNA fragment that binds to eukaryotic ribosomes. Nature. 1975; 256(5519):624-8. DOI: 10.1038/256624a0. View

20.

Briand J, Jonard G, Guilley H, Richards K, Hirth L . Nucleotide sequence (n=159) of the amino-acid-accepting 3'-OH extremity of turnip-yellow-mosaic-virus RNA and the last portion of its coat-protein cistron. Eur J Biochem. 1977; 72(3):453-63. DOI: 10.1111/j.1432-1033.1977.tb11269.x. View