Kinetics of C-phycocyanin Reaction with Hypochlorite

Overview

Journal J Protein Chem

Specialties Biochemistry
Chemistry

Date 2000 Aug 17

PMID 10945439

Citations 6

Authors

C Romay

R Gonzalez

M Pizarro

E Lissi

Affiliations

Soon will be listed here.

Abstract

Hydrochlorous acid bleaches c-phycocyanin visible absorbance with a second-order rate constant (pH 7.4) of 1.3x10(3) M(-1) s(-1). In excess of protein, ca. 0.16 bilin moieties are disrupted by each reacted HOCl molecule. This indicates that the main reaction takes place at the apoprotein level, with a total rate constant (in monomeric units concentration) of 2.5x10(4) M(-1) s(-1). This rate constant is too low to provide protection to other biomolecules under physiological conditions. The reported antiinflammatory properties of phycocyanin are not then related to the removal of HOCl. On the other hand, the rather slow reaction rate with HOCI could be beneficial to its role as antiinflammatory agent since it will allow the protein to maintain its integrity at the inflammation locus.

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References

Arnhold J, Mueller S, Arnold K, Sonntag K . Mechanisms of inhibition of chemiluminescence in the oxidation of luminol by sodium hypochlorite. J Biolumin Chemilumin. 1993; 8(6):307-13. DOI: 10.1002/bio.1170080604. View

Arnhold J, Hammerschmidt S, Wagner M, Mueller S, Arnold K, Grimm E . On the action of hypochlorite on human serum albumin. Biomed Biochim Acta. 1990; 49(10):991-7. View

Lissi E, Pizarro M, Aspee A, Romay C . Kinetics of phycocyanine bilin groups destruction by peroxyl radicals. Free Radic Biol Med. 2000; 28(7):1051-5. DOI: 10.1016/s0891-5849(00)00193-3. View

Vadiraja B, Gaikwad N, Madyastha K . Hepatoprotective effect of C-phycocyanin: protection for carbon tetrachloride and R-(+)-pulegone-mediated hepatotoxicty in rats. Biochem Biophys Res Commun. 1998; 249(2):428-31. DOI: 10.1006/bbrc.1998.9149. View

Winterbourn C, van den Berg J, ROITMAN E, Kuypers F . Chlorohydrin formation from unsaturated fatty acids reacted with hypochlorous acid. Arch Biochem Biophys. 1992; 296(2):547-55. DOI: 10.1016/0003-9861(92)90609-z. View

Folkes L, Candeias L, Wardman P . Kinetics and mechanisms of hypochlorous acid reactions. Arch Biochem Biophys. 1995; 323(1):120-6. DOI: 10.1006/abbi.1995.0017. View

Pereira W, Hoyano Y, Summons R, Bacon V, Duffield A . Chlorination studies. II. The reaction of aqueous hypochlorous acid with alpha-amino acids and dipeptides. Biochim Biophys Acta. 1973; 313(1):170-80. DOI: 10.1016/0304-4165(73)90198-0. View

Tapia G, Galetovic A, Lemp E, Pino E, Lissi E . Singlet oxygen-mediated photobleaching of the prosthetic group in hemoglobins and c-phycocyanin. Photochem Photobiol. 1999; 70(4):499-504. View

Romay C, Ledon N, Gonzalez R . Further studies on anti-inflammatory activity of phycocyanin in some animal models of inflammation. Inflamm Res. 1998; 47(8):334-8. DOI: 10.1007/s000110050338. View

10.

Gonzalez R, Rodriguez S, Romay C, Ancheta O, Gonzalez A, Armesto J . Anti-inflammatory activity of phycocyanin extract in acetic acid-induced colitis in rats. Pharmacol Res. 1999; 39(1):55-9. View

11.

Winterbourn C . Comparative reactivities of various biological compounds with myeloperoxidase-hydrogen peroxide-chloride, and similarity of the oxidant to hypochlorite. Biochim Biophys Acta. 1985; 840(2):204-10. DOI: 10.1016/0304-4165(85)90120-5. View

12.

Aruoma O, Halliwell B . Action of hypochlorous acid on the antioxidant protective enzymes superoxide dismutase, catalase and glutathione peroxidase. Biochem J. 1987; 248(3):973-6. PMC: 1148647. DOI: 10.1042/bj2480973. View

13.

Duerring M, Schmidt G, Huber R . Isolation, crystallization, crystal structure analysis and refinement of constitutive C-phycocyanin from the chromatically adapting cyanobacterium Fremyella diplosiphon at 1.66 A resolution. J Mol Biol. 1991; 217(3):577-92. DOI: 10.1016/0022-2836(91)90759-y. View

14.

Aruoma O . Scavenging of hypochlorous acid by carvedilol and ebselen in vitro. Gen Pharmacol. 1997; 28(2):269-72. DOI: 10.1016/s0306-3623(96)00232-7. View

15.

Schirmer T, Huber R, Schneider M, Bode W, Miller M, Hackert M . Crystal structure analysis and refinement at 2.5 A of hexameric C-phycocyanin from the cyanobacterium Agmenellum quadruplicatum. The molecular model and its implications for light-harvesting. J Mol Biol. 1986; 188(4):651-76. DOI: 10.1016/s0022-2836(86)80013-4. View

16.

Koppenol W . Thermodynamic considerations on the formation of reactive species from hypochlorite, superoxide and nitrogen monoxide. Could nitrosyl chloride be produced by neutrophils and macrophages?. FEBS Lett. 1994; 347(1):5-8. DOI: 10.1016/0014-5793(94)00494-3. View

17.

Marquez L, Dunford H . Chlorination of taurine by myeloperoxidase. Kinetic evidence for an enzyme-bound intermediate. J Biol Chem. 1994; 269(11):7950-6. View

18.

Rimbau V, Camins A, Romay C, Gonzalez R, Pallas M . Protective effects of C-phycocyanin against kainic acid-induced neuronal damage in rat hippocampus. Neurosci Lett. 2000; 276(2):75-8. DOI: 10.1016/s0304-3940(99)00792-2. View

19.

Haenen G, Bast A . Scavenging of hypochlorous acid by lipoic acid. Biochem Pharmacol. 1991; 42(11):2244-6. DOI: 10.1016/0006-2952(91)90363-a. View

20.

Bernofsky C . Nucleotide chloramines and neutrophil-mediated cytotoxicity. FASEB J. 1991; 5(3):295-300. DOI: 10.1096/fasebj.5.3.1848195. View