Antioxidant Properties of Alpha-crystallin

Overview

Journal J Protein Chem

Specialties Biochemistry
Chemistry

Date 2001 Sep 22

PMID 11565898

Citations 3

Authors

D Manzanares

C Bauby

R De La Pena

J C Garcia

R Sanchez

S Martinez

C H Romay

E Pino

E A Lissi

Affiliations

Soon will be listed here.

Abstract

alpha-Crystallin is a major chaperone lens protein to which has been ascribed antioxidant functions. In the present work we have evaluated the antioxidant and free radical scavenging properties of bovine alpha-crystallin in a series of in vitro models: zimosan-induced, luminol-enhanced chemiluminescence response of polymorphonuclear leukocytes, the autoxidation of brain homogenate, bleaching of 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)-derived radical cations, trapping of peroxyl radicals, and reactivity toward hypochloric acid. In all these systems, the reactivity of alpha-crystallin is higher than or similar to that of bovine serum albumin. It is concluded that, given the high concentrations of ol-crystallin in the lenses, its capacity to interact with free radicals and to remove hypochlorous acid could contribute to the maintenance of the lens functionality.

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References

Zhang Z, Zeng J, Yin H, Tang D, Borchman D, Paterson C . Membrane lipid alpha-crystallin interaction and membrane Ca2+ -ATPase activities. Curr Eye Res. 1999; 18(1):56-61. DOI: 10.1076/ceyr.18.1.56.5387. View

Atanasiu R, Stea D, Mateescu M, Vergely C, Dalloz F, Briot F . Direct evidence of caeruloplasmin antioxidant properties. Mol Cell Biochem. 1999; 189(1-2):127-35. DOI: 10.1023/a:1006945713860. View

Hipkiss A, Worthington V, Himsworth D, HERWIG W . Protective effects of carnosine against protein modification mediated by malondialdehyde and hypochlorite. Biochim Biophys Acta. 1998; 1380(1):46-54. DOI: 10.1016/s0304-4165(97)00123-2. View

Lissi E, Faure M, Clavero N . Effect of additives on the inactivation of lysozyme mediated by free radicals produced in the thermolysis of 2,2'-azo-bis-(2-amidinopropane). Free Radic Res Commun. 1991; 14(5-6):373-84. DOI: 10.3109/10715769109093426. View

Halliwell B, Gutteridge J . The antioxidants of human extracellular fluids. Arch Biochem Biophys. 1990; 280(1):1-8. DOI: 10.1016/0003-9861(90)90510-6. View

Lindena J, Burkhardt H, Dwenger A . Mechanisms of non-opsonized zymosan-induced and luminol-enhanced chemiluminescence in whole blood and isolated phagocytes. J Clin Chem Clin Biochem. 1987; 25(11):765-78. DOI: 10.1515/cclm.1987.25.11.765. View

Smith J, Jiang X, Abraham E . Identification of hydrogen peroxide oxidation sites of alpha A- and alpha B-crystallins. Free Radic Res. 1997; 26(2):103-11. DOI: 10.3109/10715769709097789. View

Wang K, Spector A . Alpha-crystallin can act as a chaperone under conditions of oxidative stress. Invest Ophthalmol Vis Sci. 1995; 36(2):311-21. View

Bose S, Mandal K, Chakrabarti B . Sensitizer-induced conformational changes in lens crystallin--II. Photodynamic action of riboflavin on bovine alpha-crystallin. Photochem Photobiol. 1986; 43(5):525-8. DOI: 10.1111/j.1751-1097.1986.tb09530.x. View

10.

Romay C, Pascual C, Lissi E . The reaction between ABTS radical cation and antioxidants and its use to evaluate the antioxidant status of serum samples. Braz J Med Biol Res. 1996; 29(2):175-83. View

11.

Hawkins C, Davies M . Hypochlorite-induced damage to proteins: formation of nitrogen-centred radicals from lysine residues and their role in protein fragmentation. Biochem J. 1998; 332 ( Pt 3):617-25. PMC: 1219520. DOI: 10.1042/bj3320617. View

12.

Cao G, Alessio H, Cutler R . Oxygen-radical absorbance capacity assay for antioxidants. Free Radic Biol Med. 1993; 14(3):303-11. DOI: 10.1016/0891-5849(93)90027-r. View

13.

Cherian M, Smith J, Jiang X, Abraham E . Influence of protein-glutathione mixed disulfide on the chaperone-like function of alpha-crystallin. J Biol Chem. 1997; 272(46):29099-103. DOI: 10.1074/jbc.272.46.29099. View

14.

Ugarte R, Edwards A, Diez M, Valenzuela A, Silva E . Riboflavin-photosensitized anaerobic modification of rat lens proteins. A correlation with age-related changes. J Photochem Photobiol B. 1992; 13(2):161-8. DOI: 10.1016/1011-1344(92)85055-y. View

15.

Preville X, Salvemini F, Giraud S, Chaufour S, Paul C, Stepien G . Mammalian small stress proteins protect against oxidative stress through their ability to increase glucose-6-phosphate dehydrogenase activity and by maintaining optimal cellular detoxifying machinery. Exp Cell Res. 1999; 247(1):61-78. DOI: 10.1006/excr.1998.4347. View

16.

Campos A, Lissi E, Vergara C, Lanio M, Alvarez C, Pazos I . Kinetics and mechanism of St I modification by peroxyl radicals. J Protein Chem. 1999; 18(3):297-306. DOI: 10.1023/a:1021087312176. View

17.

Horwitz J . Alpha-crystallin can function as a molecular chaperone. Proc Natl Acad Sci U S A. 1992; 89(21):10449-53. PMC: 50356. DOI: 10.1073/pnas.89.21.10449. View

18.

Derham B, Harding J . Alpha-crystallin as a molecular chaperone. Prog Retin Eye Res. 1999; 18(4):463-509. DOI: 10.1016/s1350-9462(98)00030-5. View

19.

Ortwerth B, James H . Lens proteins block the copper-mediated formation of reactive oxygen species during glycation reactions in vitro. Biochem Biophys Res Commun. 1999; 259(3):706-10. DOI: 10.1006/bbrc.1999.0841. View

20.

Wolff S, Wang G, Spector A . Pro-oxidant activation of ocular reductants. 1. Copper and riboflavin stimulate ascorbate oxidation causing lens epithelial cytotoxicity in vitro. Exp Eye Res. 1987; 45(6):777-89. DOI: 10.1016/s0014-4835(87)80095-7. View