Site Specific RecA--independent Recombination Between Bacterial Plasmids: Involvement of Palindromes at the Recombinational Loci

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1975 Apr 1

PMID 1093180

Citations 83

Authors

D J Kopecko

S N Cohen

Affiliations

Soon will be listed here.

Abstract

A recA-independent recombinational event is described which results in insertion of an entire plasmid genome at a unique site of another plasmid, and coincident excision of a precisely defined DNA segment originally present at the point of the insertion. The resulting recombinant molecules subsequently can undergo site-specific translocation of their component segments or inversion of their original DNA sequence orientation. The events observed entail nonreciprocal exchange of genetic material, and involve a discrete nucleotide sequences that is duplicated in rotationally symmetrical reverse orientation on plasmid DNA (i.e., inverted repeat; palindrome).

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