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Simple and Reliable Multiplex PCR Assay for Surveillance Isolates of Vancomycin-resistant Enterococci

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 2000 Aug 2
PMID 10921985
Citations 100
Authors
R Kariyama
R Mitsuhata
J W Chow
D B Clewell
H Kumon
Affiliations
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Abstract

Conditions have been optimized for the use of a multiplex PCR for the detection of vancomycin-resistant enterococci in nosocomial surveillance specimens. Seven primer sets targeting the genes vanA, vanB, vanC1, vanC2/C3 Enterococcus faecalis-specific, Enterococcus faecium-specific, and rrs (16S rRNA) were used in one reaction tube. The PCR method developed in the present study is simple and reliable for the rapid characterization of vancomycin-resistant enterococci.

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