Role of Ribosome Recycling Factor (RRF) in Translational Coupling

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2000 Jul 19

PMID 10899132

Citations 33

Authors

Y Inokuchi

A Hirashima

Y Sekine

L Janosi

A Kaji

Affiliations

Soon will be listed here.

Abstract

RNA phage GA coat and lysis protein expression are translationally coupled through an overlapping termination and initiation codon UAAUG. Essential for this coupling are the proximity of the termination codon of the upstream coat gene to the initiation codon of the lysis gene (either a <3 nucleotide separation or physical closeness through a possible hairpin structure) but not the Shine-Dalgarno sequence. This suggests that the ribosomes completing the coat gene translation are exclusively responsible for translation of the lysis gene. Inactivation of ribosome recycling factor (RRF), which normally releases ribosomes at the termination codon, did not influence the expression of the reporter gene fused to the lysis gene. This suggests the possibility that RRF may not release ribosomes from the junction UAAUG. However, RRF is essential for correct ribosomal recognition of the AUG codon as the initiation site for the lysis gene.

Citing Articles

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Role of ribosome recycling factor in natural termination and translational coupling as a ribosome releasing factor.

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Ribosome recycling is not critical for translational coupling in .

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