» Articles » PMID: 10887092

Notch Signaling Regulates the Pattern of Auditory Hair Cell Differentiation in Mammals

Overview
Journal Development
Specialty Biology
Date 2000 Jul 11
PMID 10887092
Citations 58
Authors
Affiliations
Soon will be listed here.
Abstract

The development of the mammalian cochlea is an example of patterning in the peripheral nervous system. Sensory hair cells and supporting cells in the cochlea differentiate via regional and cell fate specification. The Notch signaling components shows both distinct and overlapping expression patterns of Notch1 receptor and its ligands Jagged1 (Jag1) and Jagged2 (Jag2) in the developing auditory epithelium of the rat. On embryonic day 16 (E16), many precursor cells within the Kölliker's organ immunostained for the presence of both Notch1 and Jag1, while the area of hair cell precursors did not express either Notch1 and Jag1. During initial events of hair cell differentiation between E18 and birth, Notch1 and Jag1 expression predominated in supporting cells and Jag2 in nascent hair cells. Early after birth, Jag2 expression decreased in hair cells while the pattern of Notch1 expression now included both supporting cells and hair cells. We show that the normal pattern of hair cell differentiation is disrupted by alteration of Notch signaling. A decrease of either Notch1 or Jag1 expression by antisense oligonucleotides in cultures of the developing sensory epithelium resulted in an increase in the number of hair cells. Our data suggest that the Notch1 signaling pathway is involved in a complex interplay between the consequences of different ligand-Notch1 combinations during cochlear morphogenesis and the phases of hair cell differentiation.

Citing Articles

is Required to Maintain Supporting Cell Identity and Vestibular Function during Maturation of the Mammalian Balance Organs.

Heffer A, Lee C, Mayernik J, Holt J, Kiernan A J Neurosci. 2025; 45(9).

PMID: 39779370 PMC: 11867012. DOI: 10.1523/JNEUROSCI.1365-24.2024.


is required to maintain supporting cell identity and vestibular function during maturation of the mammalian balance organs.

Heffer A, Lee C, Holt J, Kiernan A bioRxiv. 2024; .

PMID: 38948821 PMC: 11212955. DOI: 10.1101/2024.06.21.600098.


Localization of cadherins in the postnatal cochlear epithelium and their relation to space formation.

Beaulac H, Munnamalai V Dev Dyn. 2024; 253(8):771-780.

PMID: 38264972 PMC: 11266531. DOI: 10.1002/dvdy.692.


Genetic and pharmacologic alterations of claudin9 levels suffice to induce functional and mature inner hair cells.

Chen Y, Lee J, Li J, Park S, Perez Flores M, Peguero B bioRxiv. 2023; .

PMID: 37873357 PMC: 10592694. DOI: 10.1101/2023.10.08.561387.


SoxC transcription factors shape the epigenetic landscape to establish competence for sensory differentiation in the mammalian organ of Corti.

Wang X, Llamas J, Trecek T, Shi T, Tao L, Makmura W Proc Natl Acad Sci U S A. 2023; 120(34):e2301301120.

PMID: 37585469 PMC: 10450657. DOI: 10.1073/pnas.2301301120.