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Nitration of Manganese Superoxide Dismutase in Cerebrospinal Fluids is a Marker for Peroxynitrite-mediated Oxidative Stress in Neurodegenerative Diseases

Overview
Journal Ann Neurol
Specialty Neurology
Date 2000 Apr 13
PMID 10762167
Citations 31
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Abstract

Peroxynitrite can nitrate tyrosine residues of proteins. We examined nitrotyrosine-containing proteins in cerebrospinal fluid of 66 patients with neurogenic disease by immunoblot analysis. Nitrated tyrosine residue-containing protein was observed in the cerebrospinal fluid and was concluded to be manganese superoxide dismutase (Mn-SOD). The nitrated Mn-SOD level was strikingly elevated in amyotrophic lateral sclerosis patients and was slightly increased in Alzheimer's and Parkinson's disease patients, whereas an elevated Mn-SOD level was observed only in progressive supranuclear palsy group.

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