Identification of Morphologically Similar Rhodnius Species (Hemiptera: Reduviidae: Triatominae) by Electrophoresis of Salivary Heme Proteins
Overview
Affiliations
We examined intraspecific variability in the genus Rhodnius using starch gel electrophoresis of salivary heme proteins. Salivary protein profiles of 8 Rhodnius species (R. prolixus, R. robustus, R. neglectus, R. nasutus, R. ecuadoriensis, R. pallescens, R. pictipes, and R. domesticus) were compared. All species could be distinguished by this technique. The greatest protein polymorphism was found in R. ecuadoriensis, R. nasutus, R. robustus, and R. pictipes, followed by R. prolixus, R. neglectus, R. pallescens, and R. domesticus. This approach was able to distinguish R. prolixus from R. robustus and R. neglectus from R. nasutus, species with extreme phenotypical similarity.
Wagner I, Koch N, Sarsby J, White N, Price T, Jones S Open Biol. 2020; 10(11):200196.
PMID: 33234068 PMC: 7729031. DOI: 10.1098/rsob.200196.
Meneses A, Rodriguez C, Suarez Y, Carranza J, Vallejo G Biomedica. 2020; 40(2):404-411.
PMID: 32673466 PMC: 7505504. DOI: 10.7705/biomedica.4992.
Falcone R, Ribeiro A, de Oliveira J, Mendonca V, Graminha M, Aristeu da Rosa J Rev Soc Bras Med Trop. 2020; 53:e20190503.
PMID: 32267457 PMC: 7156257. DOI: 10.1590/0037-8682-0503-2019.
Paim R, Nascimento B, Nascimento A, Pacheco D, Soares A, Araujo R Sci Rep. 2017; 7(1):16036.
PMID: 29167493 PMC: 5700186. DOI: 10.1038/s41598-017-16097-8.
A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease.
Santiago P, Assumpcao T, de Araujo C, Bastos I, Neves D, da Silva I PLoS Negl Trop Dis. 2016; 10(4):e0004581.
PMID: 27129103 PMC: 4851354. DOI: 10.1371/journal.pntd.0004581.