» Articles » PMID: 10744773

Microsomal Triglyceride Transfer Protein Expression During Mouse Development

Overview
Journal J Lipid Res
Publisher Elsevier
Specialty Biochemistry
Date 2000 Apr 1
PMID 10744773
Citations 55
Authors
Affiliations
Soon will be listed here.
Abstract

Feto-maternal transfer of lipophilic nutrients is an important factor in the normal development of the fetus and may be mediated by lipoproteins as carriers of these nutrients. Two proteins that may be important in this process are apolipoprotein B (apoB, the major structural protein of secreted lipoproteins) and microsomal triglyceride transfer protein (MTP) whose normal activity is required for the secretion of apoB-containing lipoproteins. Although no abnormalities of conception and embryonic lethality are known in humans who inherit genetic deficiencies of either of these proteins, homozygous mice bearing knockouts of either apoB or MTP show early embryonic lethality. To characterize the ontogeny of MTP expression during embryonic mouse development, we have used in situ hybridization to characterize the pattern of expression. By using microwave heating of tissue sections to optimize hybridization, we show that there is robust MTP expression in the yolk sac tissues followed by expression in the primordial liver cell nests as early as day 9 post-coitum (E9.5). Intestinal expression is detected around E12.5 and attains full adult expression patterns by E14.5. No expression in any other tissues was observed, including developing heart, kidney, placenta, and maternal decidua. Thus the pattern of MTP expression is compatible with a role in the transfer of lipophilic nutrients from the yolk sac, prior to hepatic development and to the liver, once the circulatory system has been established.

Citing Articles

A myocardin-adjacent lncRNA balances SRF-dependent gene transcription in the heart.

Anderson D, Anderson K, Nelson B, McAnally J, Bezprozvannaya S, Shelton J Genes Dev. 2021; 35(11-12):835-840.

PMID: 33985971 PMC: 8168554. DOI: 10.1101/gad.348304.121.


The nuclear envelope protein Net39 is essential for muscle nuclear integrity and chromatin organization.

Ramirez-Martinez A, Zhang Y, Chen K, Kim J, Cenik B, McAnally J Nat Commun. 2021; 12(1):690.

PMID: 33514739 PMC: 7846557. DOI: 10.1038/s41467-021-20987-x.


Ankmy2 Prevents Smoothened-Independent Hyperactivation of the Hedgehog Pathway via Cilia-Regulated Adenylyl Cyclase Signaling.

Somatilaka B, Hwang S, Palicharla V, White K, Badgandi H, Shelton J Dev Cell. 2020; 54(6):710-726.e8.

PMID: 32702291 PMC: 9042708. DOI: 10.1016/j.devcel.2020.06.034.


The G protein-coupled receptor Gpr161 regulates forelimb formation, limb patterning and skeletal morphogenesis in a primary cilium-dependent manner.

Hwang S, White K, Somatilaka B, Shelton J, Richardson J, Mukhopadhyay S Development. 2017; 145(1).

PMID: 29222391 PMC: 5825871. DOI: 10.1242/dev.154054.


KLHL41 stabilizes skeletal muscle sarcomeres by nonproteolytic ubiquitination.

Ramirez-Martinez A, Cenik B, Bezprozvannaya S, Chen B, Bassel-Duby R, Liu N Elife. 2017; 6.

PMID: 28826497 PMC: 5589419. DOI: 10.7554/eLife.26439.