Multiplication of Antenna Genes As a Major Adaptation to Low Light in a Marine Prokaryote

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2000 Mar 22

PMID 10725393

Citations 29

Authors

L Garczarek

W R Hess

J Holtzendorff

G W van der Staay

F Partensky

Affiliations

Soon will be listed here.

Abstract

Two ecotypes of the prokaryote Prochlorococcus adapted to distinct light niches in the ocean have been described recently. These ecotypes are characterized by their different (divinyl-) chlorophyll (Chl) a to Chl b ratios and 16S rRNA gene signatures, as well as by their significantly distinct irradiance optima for growth and photosynthesis [Moore, L. R., Rocap, G. & Chisholm, S. W. (1998) Nature (London) 393, 464-467]. However, the molecular basis of their physiological differences remained, so far, unexplained. In this paper, we show that the low-light-adapted Prochlorococcus strain SS120 possesses a gene family of seven transcribed genes encoding different Chl a/b-binding proteins (Pcbs). In contrast, Prochlorococcus sp. MED4, a high-light-adapted ecotype, possesses a single pcb gene. The presence of multiple antenna genes in another low-light ecotype (NATL2a), but not in another high-light ecotype (TAK9803-2), is demonstrated. Thus, the multiplication of pcb genes appears as a key factor in the capacity of deep Prochlorococcus populations to survive at extremely low photon fluxes.

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References

La Roche J, van der Staay G, Partensky F, Ducret A, Aebersold R, Li R . Independent evolution of the prochlorophyte and green plant chlorophyll a/b light-harvesting proteins. Proc Natl Acad Sci U S A. 1996; 93(26):15244-8. PMC: 26388. DOI: 10.1073/pnas.93.26.15244. View

Moore L, Rocap G, Chisholm S . Physiology and molecular phylogeny of coexisting Prochlorococcus ecotypes. Nature. 1998; 393(6684):464-7. DOI: 10.1038/30965. View

Kotani H, Tabata S . LESSONS FROM SEQUENCING OF THE GENOME OF A UNICELLULAR CYANOBACTERIUM, SYNECHOCYSTIS SP. PCC6803. Annu Rev Plant Physiol Plant Mol Biol. 2004; 49:151-171. DOI: 10.1146/annurev.arplant.49.1.151. View

Urbach E, Scanlan D, Distel D, Waterbury J, Chisholm S . Rapid diversification of marine picophytoplankton with dissimilar light-harvesting structures inferred from sequences of Prochlorococcus and Synechococcus (Cyanobacteria). J Mol Evol. 1998; 46(2):188-201. DOI: 10.1007/pl00006294. View

Bustos S, Golden S . Light-regulated expression of the psbD gene family in Synechococcus sp. strain PCC 7942: evidence for the role of duplicated psbD genes in cyanobacteria. Mol Gen Genet. 1992; 232(2):221-30. DOI: 10.1007/BF00280000. View

Partensky F, Hess W, Vaulot D . Prochlorococcus, a marine photosynthetic prokaryote of global significance. Microbiol Mol Biol Rev. 1999; 63(1):106-27. PMC: 98958. DOI: 10.1128/MMBR.63.1.106-127.1999. View

Hess W, Weihe A, Partensky F, Vaulot D . Characterization of the single psbA gene of Prochlorococcus marinus CCMP 1375 (Prochlorophyta). Plant Mol Biol. 1995; 27(6):1189-96. DOI: 10.1007/BF00020892. View

Schaefer M, Golden S . Differential expression of members of a cyanobacterial psbA gene family in response to light. J Bacteriol. 1989; 171(7):3973-81. PMC: 210150. DOI: 10.1128/jb.171.7.3973-3981.1989. View

Smith S, Overbeek R, Woese C, Gilbert W, Gillevet P . The genetic data environment an expandable GUI for multiple sequence analysis. Comput Appl Biosci. 1994; 10(6):671-5. DOI: 10.1093/bioinformatics/10.6.671. View

10.

West N, Scanlan D . Niche-partitioning of Prochlorococcus populations in a stratified water column in the eastern North Atlantic Ocean. Appl Environ Microbiol. 1999; 65(6):2585-91. PMC: 91382. DOI: 10.1128/AEM.65.6.2585-2591.1999. View

11.

Durnford D, Deane J, Tan S, McFadden G, Gantt E, Green B . A phylogenetic assessment of the eukaryotic light-harvesting antenna proteins, with implications for plastid evolution. J Mol Evol. 1999; 48(1):59-68. DOI: 10.1007/pl00006445. View

12.

van der Staay G, Yurkova N, Green B . The 38 kDa chlorophyll a/b protein of the prokaryote Prochlorothrix hollandica is encoded by a divergent pcb gene. Plant Mol Biol. 1998; 36(5):709-16. DOI: 10.1023/a:1005930210515. View

13.

Strehl B, Holtzendorff J, Partensky F, Hess W . A small and compact genome in the marine cyanobacterium Prochlorococcus marinus CCMP 1375: lack of an intron in the gene for tRNA(Leu)(UAA) and a single copy of the rRNA operon. FEMS Microbiol Lett. 1999; 181(2):261-6. DOI: 10.1111/j.1574-6968.1999.tb08853.x. View