Nucleotide Sequence of the Bla(RTG-2) (CARB-5) Gene and Phylogeny of a New Group of Carbenicillinases

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2000 Mar 18

PMID 10722515

Citations 15

Authors

D Choury

M F Szajnert

M L Joly-Guillou

K Azibi

M Delpech

G Paul

Affiliations

Soon will be listed here.

Abstract

We determined the nucleotide sequence of the bla gene for the Acinetobacter calcoaceticus beta-lactamase previously described as CARB-5. Alignment of the deduced amino acid sequence with those of known beta-lactamases revealed that CARB-5 possesses an RTG triad in box VII, as described for the Proteus mirabilis GN79 enzyme, instead of the RSG consensus characteristic of the other carbenicillinases. Phylogenetic studies showed that these RTG enzymes constitute a new, separate group, possibly ancestors of the carbenicillinase family.

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