Cytokine MRNA Expression in Mucosal Biopsies from German Shepherd Dogs with Small Intestinal Enteropathies

Overview

Journal Dig Dis Sci

Specialty Gastroenterology

Date 2000 Mar 1

PMID 10695606

Citations 26

Authors

A J German

C R Helps

E J Hall

M J Day

Affiliations

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Abstract

German shepherd dogs (GSD) are predisposed to enteropathies such as inflammatory bowel disease (IBD) and small intestinal bacterial overgrowth (SIBO). The present study examined the role of cytokines in the immunopathogenesis of both conditions. Duodenal mucosal biopsies were taken from GSDs with small intestinal enteropathies (group 1; N = 16) or control dogs (group 2, N = 12). IL-2, IL-4, IL-5, IL-10, IL-12p40, IFN-gamma, TNF-alpha, and TGF-beta1 mRNA expression was determined by semiquantitative reverse transcriptase polymerase chain reaction. IL-2, IL-5, IL-12p40, TNF-alpha, and TGF-beta1 mRNA expression in group 1 dogs was significantly greater than in group 2 dogs (all P<0.01), but there were no significant differences between dogs with IBD or SIBO. Further, antibiotic treatment in five dogs with SIBO, resulted in reduced TNF-alpha and TGF-beta1 mRNA expression (P<0.05). Such alterations in cytokine mRNA expression suggest heightened immune responses within the duodenal mucosa in GSDs with either SIBO or IBD.

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References

Viney J . Altering cytokine soups: a recipe for inflammatory bowel disease?. Gut. 1998; 42(5):607-8. PMC: 1727105. DOI: 10.1136/gut.42.5.607. View

Desreumaux P, Brandt E, Gambiez L, Emilie D, Geboes K, Klein O . Distinct cytokine patterns in early and chronic ileal lesions of Crohn's disease. Gastroenterology. 1997; 113(1):118-26. DOI: 10.1016/s0016-5085(97)70116-1. View

Batt R, Needham J, Carter M . Bacterial overgrowth associated with a naturally occurring enteropathy in the German shepherd dog. Res Vet Sci. 1983; 35(1):42-6. View

Niessner M, Volk B . Altered Th1/Th2 cytokine profiles in the intestinal mucosa of patients with inflammatory bowel disease as assessed by quantitative reversed transcribed polymerase chain reaction (RT-PCR). Clin Exp Immunol. 1995; 101(3):428-35. PMC: 1553229. DOI: 10.1111/j.1365-2249.1995.tb03130.x. View

Papasouliotis K, Sparkes A, Werrett G, Egan K, Gruffydd-Jones T . Assessment of the bacterial flora of the proximal part of the small intestine in healthy cats, and the effect of sample collection method. Am J Vet Res. 1998; 59(1):48-51. View

De Boissieu D, Chaussain M, Badoual J, Raymond J, Dupont C . Small-bowel bacterial overgrowth in children with chronic diarrhea, abdominal pain, or both. J Pediatr. 1996; 128(2):203-7. DOI: 10.1016/s0022-3476(96)70390-6. View

Brandwein S, McCabe R, Cong Y, Waites K, Ridwan B, Dean P . Spontaneously colitic C3H/HeJBir mice demonstrate selective antibody reactivity to antigens of the enteric bacterial flora. J Immunol. 1997; 159(1):44-52. View

Simpson K . Small intestinal bacterial overgrowth. J Am Vet Med Assoc. 1994; 205(3):405-7. View

Batt R, Rutgers H, Sancak A . Enteric bacteria: friend or foe?. J Small Anim Pract. 1996; 37(6):261-7. DOI: 10.1111/j.1748-5827.1996.tb02376.x. View

10.

Fiocchi C, Fukushima K, Strong S, Ina K . Pitfalls in cytokine analysis in inflammatory bowel disease. Aliment Pharmacol Ther. 1996; 10 Suppl 2:63-9; discussion 70-1. DOI: 10.1046/j.1365-2036.1996.22164022.x. View

11.

Humbert P, Treffel P, Chapuis J, Buchet S, Derancourt C, Agache P . The tetracyclines in dermatology. J Am Acad Dermatol. 1991; 25(4):691-7. DOI: 10.1016/0190-9622(91)70255-z. View

12.

Hinton M, Hampson D, Hampson E, LINTON A . The effects of oxytetracycline on the intestinal Escherichia coli flora of newly weaned pigs. J Hyg (Lond). 1985; 95(1):77-85. PMC: 2129504. DOI: 10.1017/s0022172400062306. View

13.

Veress B, Lofberg R, Bergman L . Microscopic colitis syndrome. Gut. 1995; 36(6):880-6. PMC: 1382626. DOI: 10.1136/gut.36.6.880. View

14.

Chaffins M, Collison D, Fivenson D . Treatment of pemphigus and linear IgA dermatosis with nicotinamide and tetracycline: a review of 13 cases. J Am Acad Dermatol. 1993; 28(6):998-1000. DOI: 10.1016/s0190-9622(08)80651-2. View

15.

Batt R, Barnes A, Rutgers H, Carter S . Relative IgA deficiency and small intestinal bacterial overgrowth in German shepherd dogs. Res Vet Sci. 1991; 50(1):106-11. DOI: 10.1016/0034-5288(91)90062-s. View

16.

Rutgers H, Batt R, Elwood C, Lamport A . Small intestinal bacterial overgrowth in dogs with chronic intestinal disease. J Am Vet Med Assoc. 1995; 206(2):187-93. View

17.

Rutgers H, Batt R, Proud F, Sorensen S, Elwood C, Petrie G . Intestinal permeability and function in dogs with small intestinal bacterial overgrowth. J Small Anim Pract. 1996; 37(9):428-34. DOI: 10.1111/j.1748-5827.1996.tb02443.x. View

18.

Letterio J, Roberts A . Regulation of immune responses by TGF-beta. Annu Rev Immunol. 1998; 16:137-61. DOI: 10.1146/annurev.immunol.16.1.137. View

19.

Jawhari A, Talbot I . Microscopic, lymphocytic and collagenous colitis. Histopathology. 1996; 29(2):101-10. DOI: 10.1046/j.1365-2559.1996.d01-498.x. View

20.

Schijns V, Wierda C, van Dam E, Vahlenkamp T, Horzinek M . Molecular cloning of cat interleukin-4. Immunogenetics. 1995; 42(5):434-5. DOI: 10.1007/BF00179411. View