Cyclooxygenase-2 and 5-lipoxygenase in Dogs with Chronic Enteropathies

Overview

Journal J Vet Intern Med

Specialties General Medicine
Veterinary Medicine

Date 2014 Oct 2

PMID 25269796

Citations 11

Authors

S D Dumusc

E C Ontsouka

M Schnyder

S Hartnack

C Albrecht

R M Bruckmaier

I A Burgener

Affiliations

Soon will be listed here.

Abstract

Background: Cyclooxygenase-2 (COX-2) is a key enzyme in the synthesis of pro-inflammatory prostaglandins and 5-lipoxygenase (5-LO) is the major source of leukotrienes. Their role in IBD has been demonstrated in humans and animal models, but not in dogs with chronic enteropathies (CCE).

Hypothesis: COX-2 and 5-LO are upregulated in dogs with CCE.

Animals: Fifteen healthy control dogs (HCD), 10 dogs with inflammatory bowel disease (IBD), and 15 dogs with food-responsive diarrhea (FRD).

Methods: Prospective study. mRNA expression of COX-2, 5-LO, IL-1b, IL-4, IL-6, TNF, IL-10 and TFG-β was evaluated by quantitative real-time RT-PCR in duodenal and colonic biopsies before and after treatment.

Results: COX-2 expression in the colon was significantly higher in IBD and FRD before and after treatment (all P < .01). IL-1b was higher in FRD in the duodenum after treatment (P = .021). TGF-β expression was significantly higher in the duodenum of HCD compared to FRD/IBD before treatment (both P < .001) and IBD after treatment (P = .012). There were no significant differences among groups and within groups before and after treatment for IL-4, IL-6, TNF, and IL-10. There was a significant correlation between COX-2 and IL-1b in duodenum and colon before treatment in FRD and IBD, whereas 5-LO correlated better with IL-6 and TNF. IL-10 and TGF-β usually were correlated.

Conclusions And Clinical Importance: COX-2 is upregulated in IBD and FRD, whereas IL-1b and TGF-β seem to be important pro- and anti-inflammatory cytokines, respectively. The use of dual COX/5-LO inhibitors could be an interesting alternative in the treatment of CCE.

Citing Articles

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Heilmann R, Csukovich G, Burgener I, Dengler F Front Vet Sci. 2023; 10:1180125.

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Crisi P, Luciani A, Di Tommaso M, Prasinou P, De Santis F, Chatgilialoglu C Animals (Basel). 2021; 11(9).

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Becher A, Suchodolski J, Steiner J, Heilmann R J Vet Diagn Invest. 2021; 33(3):516-527.

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