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A Spectrophotometric Method for Assay of Tannase Using Rhodanine

Overview
Journal Anal Biochem
Publisher Elsevier
Specialty Biochemistry
Date 2000 Feb 23
PMID 10683234
Citations 47
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Abstract

A method for assay of microbial tannase (tannin acyl hydrolase) based on the formation of chromogen between gallic acid and rhodanine is reported. Unlike the previous protocols, this method is sensitive up to gallic acid concentration of 5 nmol and has a precision of 1.7% (relative standard deviation). The assay is complete in a short time, very convenient, and reproducible.

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