Photoaffinity Labeling and Quaternary Structure of the Acetylcholine Receptor from Torpedo Californica

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1976 Aug 1

PMID 1066671

Citations 7

Authors

F Hucho

P Layer

H R Kiefer

G Bandini

Affiliations

Soon will be listed here.

Abstract

Membrane fragments from electric tissue of Torpedo californica containing nicotinic acetylcholine receptor are composed of four different polypeptide chains with molecular weights of 40,000 (alpha), 48,000 (beta), 62,000 (gamma), and 66,000 (delta). The alpha and beta chains are still present in all and gamma and delta in some of the receptor preparations after Triton X-100 extraction and purification by affinity chromatography. All components of the receptor react covalently with the photoaffinity label 4-azido-2-nitrobenzyltrimethylammonium fluoroborate, the delta chain incorporating less of the reagent as compared to the alpha and beta chains. Agonists and antagonists containing a quaternary ammonium group protect all chains against the label; the principal neurotoxin from Naja naja siamensis protects the alpha chain only. We conclude that the alpha chain binds the neurotoxin from Naja naja, the alpha and beta chains are involved in the binding of ligands with quaternary ammonium groups, and the function of the gamma and delta chains remains to be determined.

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Immunofluorescence localization at the mammalian neuromuscular junction of the Mr 43,000 protein of Torpedo postsynaptic membranes.

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Activation and desensitization of Torpedo acetylcholine receptor: evidence for separate binding sites.

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Electron microscopy of complexes of isolated acetylcholine receptor, biotinyl-toxin, and avidin.

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