Pseudomonas Aeruginosa Pyocyanin and 1-hydroxyphenazine Inhibit Fungal Growth

Overview

Journal J Clin Pathol

Specialty Pathology

Date 1999 Nov 24

PMID 10560362

Citations 124

Authors

J R Kerr

G W Taylor

A Rutman

N Hoiby

P J COLE

R Wilson

Affiliations

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Abstract

Aim: To examine strains of Pseudomonas aeruginosa for specific antifungal factors.

Methods: Two clinical strains of P aeruginosa with strong in vitro inhibition (by cross streak assay) of Candida albicans and Aspergillus fumigatus were examined. Both strains were isolated from sputum--one from a patient with cystic fibrosis and one from a patient with bronchiectasis. Bacterial extracts were fractionated by high performance liquid chromatography and examined by ultraviolet absorbance and mass spectroscopy. Antifungal activity against C albicans and A fumigatus was determined in a well plate assay.

Results: Pyocyanin was the major antifungal agent of P aeruginosa; 1-hydroxy-phenazine also possessed activity. Pyocyanin MICs for C albicans and A fumigatus were > 64 micrograms/ml. These phenazines were active against nine other yeast species pathogenic for man. Preliminary experiments also suggested possible inhibition of yeast mycelial transformation in C albicans by pyocyanin.

Conclusions: There may be a role for pyocyanin and 1-hydroxyphenazine in the prevention of pulmonary candidiasis in patients colonised by P aeruginosa.

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