MYCN Gene Amplification. Identification of Cell Populations Containing Double Minutes and Homogeneously Staining Regions in Neuroblastoma Tumors

Overview

Journal Am J Pathol

Publisher Elsevier

Specialty Pathology

Date 1999 Nov 7

PMID 10550298

Citations 13

Authors

M Yoshimoto

S R Caminada De Toledo

E M Monteiro Caran

M T de Seixas

M L de Martino Lee

S de Campos Vieira Abib

S M Vianna

S T Schettini

J Anderson Duffles Andrade

Affiliations

Soon will be listed here.

Abstract

Neuroblastoma is the second most common solid tumor occurring in children. Amplification of the MYCN oncogene is associated with poor prognosis. To identify neuroblastoma tumors with MYCN amplification, we studied the number of copies of MYCN in interphase cells by fluorescence in situ hybridization in 20 neuroblastoma patients. MYCN amplification appeared in 7 tumor specimens. Interphase and metaphase studies showed a tumor cell population with both forms of amplification, double minutes and homogeneously staining regions, in two patients. These patients showed a smaller tumor cell subpopulation with the presence of more than one homogeneously staining region, suggesting that gene amplification was undergoing karyotype evolution.

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References

Brodeur G . Molecular basis for heterogeneity in human neuroblastomas. Eur J Cancer. 1995; 31A(4):505-10. DOI: 10.1016/0959-8049(95)00040-p. View

Hiemstra J, Schneider S, Brodeur G . High-resolution mapping of the N-myc amplicon core domain in neuroblastomas. Prog Clin Biol Res. 1994; 385:51-7. View

Brodeur G, Seeger R, Schwab M, Varmus H, Bishop J . Amplification of N-myc in untreated human neuroblastomas correlates with advanced disease stage. Science. 1984; 224(4653):1121-4. DOI: 10.1126/science.6719137. View

Brodeur G, Fong C . Molecular biology and genetics of human neuroblastoma. Cancer Genet Cytogenet. 1989; 41(2):153-74. DOI: 10.1016/0165-4608(89)90243-4. View

Schwab M, Varmus H, Bishop J, Grzeschik K, Naylor S, Sakaguchi A . Chromosome localization in normal human cells and neuroblastomas of a gene related to c-myc. Nature. 1984; 308(5956):288-91. DOI: 10.1038/308288a0. View

Brodeur G, Green A, Hayes F, Williams K, Williams D, Tsiatis A . Cytogenetic features of human neuroblastomas and cell lines. Cancer Res. 1981; 41(11 Pt 1):4678-86. View

Look A, Hayes F, Shuster J, Douglass E, Castleberry R, Bowman L . Clinical relevance of tumor cell ploidy and N-myc gene amplification in childhood neuroblastoma: a Pediatric Oncology Group study. J Clin Oncol. 1991; 9(4):581-91. DOI: 10.1200/JCO.1991.9.4.581. View

Cohen P, Seeger R, Triche T, Israel M . Detection of N-myc gene expression in neuroblastoma tumors by in situ hybridization. Am J Pathol. 1988; 131(3):391-7. PMC: 1880701. View

Cowell J . A new chromosome region possibly derived from double minutes in an in vitro transformed epithelial cell line. Cytogenet Cell Genet. 1980; 27(1):2-7. DOI: 10.1159/000131458. View

10.

Balaban-Malenbaum G, Gilbert F . Double minute chromosomes and the homogeneously staining regions in chromosomes of a human neuroblastoma cell line. Science. 1977; 198(4318):739-41. DOI: 10.1126/science.71759. View

11.

Quinn L, Moore G, Morgan R, Woods L . Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 1979; 39(12):4914-24. View

12.

Leong P, Thorner P, Yeger H, Ng K, Zhang Z, Squire J . Detection of MYCN gene amplification and deletions of chromosome 1p in neuroblastoma by in situ hybridization using routine histologic sections. Lab Invest. 1993; 69(1):43-50. View

13.

Balaban G, Gilbert F . Homogeneously staining regions in direct preparations from human neuroblastomas. Cancer Res. 1982; 42(5):1838-42. View

14.

Windle B, Wahl G . Molecular dissection of mammalian gene amplification: new mechanistic insights revealed by analyses of very early events. Mutat Res. 1992; 276(3):199-224. DOI: 10.1016/0165-1110(92)90009-x. View

15.

GEORGE D, Francke U . Homogeneously staining chromosome regions and double minutes in a mouse adrenocortical tumor cell line. Cytogenet Cell Genet. 1980; 28(4):217-26. DOI: 10.1159/000131535. View

16.

Cowell J . Chromosome abnormalities associated with salivary gland epithelial cell lines transformed in vitro and in vivo with evidence of a role for genetic imbalance in transformation. Cancer Res. 1981; 41(4):1508-17. View

17.

Cohn S, Look A, JOSHI V, Holbrook T, Salwen H, Chagnovich D . Lack of correlation of N-myc gene amplification with prognosis in localized neuroblastoma: a Pediatric Oncology Group study. Cancer Res. 1995; 55(4):721-6. View

18.

Brodeur G, Seeger R . Gene amplification in human neuroblastomas: basic mechanisms and clinical implications. Cancer Genet Cytogenet. 1986; 19(1-2):101-11. DOI: 10.1016/0165-4608(86)90377-8. View

19.

Levan A, Levan G, Mandahl N . A new chromosome type replacing the double minutes in a mouse tumor. Cytogenet Cell Genet. 1978; 20(1-6):12-23. DOI: 10.1159/000130836. View

20.

Squire , Thorner , Marrano , Parkinson , Ng , Gerrie . Identification of MYCN Copy Number Heterogeneity by Direct FISH Analysis of Neuroblastoma Preparations. Mol Diagn. 1996; 1(4):281-289. DOI: 10.1054/MODI00100281. View