5'-Terminal M-7G(5')ppp(5')G-m-p in Vivo: Identification in Reovirus Genome RNA

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1975 Feb 1

PMID 1054852

Citations 57

Authors

Y Furuichi

S Muthukrishnan

A J Shatkin

Affiliations

Soon will be listed here.

Abstract

Methylated reovirus mRNA was synthesized in vitro in the presence of S-adenosyl-L-[methyl-3H]-methionine. Viral genome double-stranded RNA that was uniformly labeled with 32-P was isolated from purified virions. The RNAs were mixed and their 5'-terminal structures compared by electrophoretic and chromatographic analyses after enzymatic digestion. Both the mRNA and the corresponding strand in the genome RNA contain m-7G(5')ppp(5')G-m-pCp, indicating that infected cells synthesize viral RNA with blocked, methylated 5' termini.

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