Ro/SSA and La/SSB Specific IgA Autoantibodies in Serum of Patients with Sjögren's Syndrome and Systemic Lupus Erythematosus

Overview

Journal Ann Rheum Dis

Specialty Rheumatology

Date 1999 Sep 24

PMID 10491361

Citations 13

Authors

N Pourmand

M Wahren-Herlenius

I Gunnarsson

E Svenungsson

B Lofstrom

Y Ioannou

D A Isenberg

C G Magnusson

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate the occurrence of IgA autoantibodies to Ro 52 kDa, Ro 60 kDa and La antigen in serum of patients with primary Sjögren's syndrome (pSS) and systemic lupus erythematosus (SLE).

Methods: Recombinant Ro 52 kDa, Ro 60 kDa and La antigens were used to analyse autoantibodies in serum from 25 patients with pSS, 30 patients with SLE and 20 controls using a semiquantitative immunoblotting approach.

Results: Among the patients with pSS, 21 (84%) had detectable IgA autoantibodies to Ro 52 kDa, 13 (52%) to Ro 60 kDa and 20 (80%) to La antigen. The corresponding results for the patients with SLE were 22 (73%), 14 (47%) and 20 (67%), respectively. No IgA autoantibodies against the three antigens were detected in 20 normal controls. A comparison of several clinical features with the titres of IgA antibodies to Ro 52 kDa, Ro 60 kDa and La, revealed a significant relation between IgA anti-Ro 52 and IgA anti-La to sicca (p< 0.05). Semiquantitative data suggest that IgG is the dominating antibody to the three antigens followed by IgM > IgA in both SLE and pSS patients. Specificity studies of IgA autoantibodies with different subfragments of Ro 52 kDa and Ro 60 kDa antigens showed that IgA antibodies did not differ from IgG and IgM in their recognition pattern.

Conclusion: These results suggest that besides IgM and IgG, IgA autoantibodies are also detected at high frequency in patients with pSS and SLE. Further studies are necessary to evaluate the contribution of these IgA autoantibodies to inflammation as well as their diagnostic value.

Citing Articles

Clinical, Radiological, and Laboratory Features of Spinal Cord Involvement in Primary Sjögren's Syndrome.

Butryn M, Neumann J, Rolfes L, Bartels C, Wattjes M, Mahmoudi N J Clin Med. 2020; 9(5).

PMID: 32423153 PMC: 7290729. DOI: 10.3390/jcm9051482.

Sex differences in clinical presentation of systemic lupus erythematosus.

Ramirez Sepulveda J, Bolin K, Mofors J, Leonard D, Svenungsson E, Jonsen A Biol Sex Differ. 2019; 10(1):60.

PMID: 31843005 PMC: 6915972. DOI: 10.1186/s13293-019-0274-2.

Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces.

Azzam K, Madenspacher J, Cain D, Lai L, Gowdy K, Rai P JCI Insight. 2017; 2(16).

PMID: 28814662 PMC: 5621910. DOI: 10.1172/jci.insight.91914.

Difference in clinical presentation between women and men in incident primary Sjögren's syndrome.

Ramirez Sepulveda J, Kvarnstrom M, Brauner S, Baldini C, Wahren-Herlenius M Biol Sex Differ. 2017; 8:16.

PMID: 28507729 PMC: 5427625. DOI: 10.1186/s13293-017-0137-7.

The Interferon-Signature of Sjögren's Syndrome: How Unique Biomarkers Can Identify Underlying Inflammatory and Immunopathological Mechanisms of Specific Diseases.

Nguyen C, Peck A Front Immunol. 2013; 4:142.

PMID: 23847613 PMC: 3701867. DOI: 10.3389/fimmu.2013.00142.

References

Hendrick J, Wolin S, Rinke J, Lerner M, Steitz J . Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleoproteins: further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells. Mol Cell Biol. 1981; 1(12):1138-49. PMC: 369740. DOI: 10.1128/mcb.1.12.1138-1149.1981. View

Halse A, Wahren-Herlenius M, Jonsson R . Ro/SS-A- and La/SS-B-reactive B lymphocytes in peripheral blood of patients with Sjögren's syndrome. Clin Exp Immunol. 1999; 115(1):208-13. PMC: 1905183. DOI: 10.1046/j.1365-2249.1999.00779.x. View

Kato N, Hoshino H, Harada F . Nucleotide sequence of 4.5S RNA (C8 or hY5) from HeLa cells. Biochem Biophys Res Commun. 1982; 108(1):363-70. DOI: 10.1016/0006-291x(82)91875-7. View

Hall R, Stachura I, Cason J, Whiteside T, Lawley T . IgA-containing circulating immune complexes in patients with igA nephropathy. Am J Med. 1983; 74(1):56-63. DOI: 10.1016/0002-9343(83)91118-x. View

Conley M, Koopman W . Serum IgA1 and IgA2 in normal adults and patients with systemic lupus erythematosus and hepatic disease. Clin Immunol Immunopathol. 1983; 26(3):390-7. DOI: 10.1016/0090-1229(83)90123-x. View

Stefano J . Purified lupus antigen La recognizes an oligouridylate stretch common to the 3' termini of RNA polymerase III transcripts. Cell. 1984; 36(1):145-54. DOI: 10.1016/0092-8674(84)90083-7. View

Elkon K, Gharavi A, Hughes G, Moutsoupoulos H . Autoantibodies in the sicca syndrome (primary Sjögren's syndrome). Ann Rheum Dis. 1984; 43(2):243-5. PMC: 1001474. DOI: 10.1136/ard.43.2.243. View

Wolin S, Steitz J . The Ro small cytoplasmic ribonucleoproteins: identification of the antigenic protein and its binding site on the Ro RNAs. Proc Natl Acad Sci U S A. 1984; 81(7):1996-2000. PMC: 345423. DOI: 10.1073/pnas.81.7.1996. View

Akerstrom B, Bjorck L . A physicochemical study of protein G, a molecule with unique immunoglobulin G-binding properties. J Biol Chem. 1986; 261(22):10240-7. View

10.

Mestecky J, McGhee J . Immunoglobulin A (IgA): molecular and cellular interactions involved in IgA biosynthesis and immune response. Adv Immunol. 1987; 40:153-245. DOI: 10.1016/s0065-2776(08)60240-0. View

11.

Bruggemann M, Williams G, Bindon C, Clark M, Walker M, Jefferis R . Comparison of the effector functions of human immunoglobulins using a matched set of chimeric antibodies. J Exp Med. 1987; 166(5):1351-61. PMC: 2189658. DOI: 10.1084/jem.166.5.1351. View

12.

Ben-Chetrit E, Chan E, Sullivan K, Tan E . A 52-kD protein is a novel component of the SS-A/Ro antigenic particle. J Exp Med. 1988; 167(5):1560-71. PMC: 2188933. DOI: 10.1084/jem.167.5.1560. View

13.

Deutscher S, Harley J, Keene J . Molecular analysis of the 60-kDa human Ro ribonucleoprotein. Proc Natl Acad Sci U S A. 1988; 85(24):9479-83. PMC: 282776. DOI: 10.1073/pnas.85.24.9479. View

14.

Ben-Chetrit E, Gandy B, Tan E, Sullivan K . Isolation and characterization of a cDNA clone encoding the 60-kD component of the human SS-A/Ro ribonucleoprotein autoantigen. J Clin Invest. 1989; 83(4):1284-92. PMC: 303819. DOI: 10.1172/JCI114013. View

15.

Gottlieb E, Steitz J . The RNA binding protein La influences both the accuracy and the efficiency of RNA polymerase III transcription in vitro. EMBO J. 1989; 8(3):841-50. PMC: 400883. DOI: 10.1002/j.1460-2075.1989.tb03445.x. View

16.

Gumpel J, Hobbs J . Serum immune globulins in Sjögren's syndrome. Ann Rheum Dis. 1970; 29(6):681-3. PMC: 1010603. DOI: 10.1136/ard.29.6.681. View

17.

Whaley K, Webb J, McAvoy B, Hughes G, Lee P, MacSween R . Sjogren's syndrome. 2. Clinical associations and immunological phenomena. Q J Med. 1973; 42(167):513-48. View

18.

Gottlieb E, Steitz J . Function of the mammalian La protein: evidence for its action in transcription termination by RNA polymerase III. EMBO J. 1989; 8(3):851-61. PMC: 400884. DOI: 10.1002/j.1460-2075.1989.tb03446.x. View

19.

Russell M, MANSA B . Complement-fixing properties of human IgA antibodies. Alternative pathway complement activation by plastic-bound, but not specific antigen-bound, IgA. Scand J Immunol. 1989; 30(2):175-83. DOI: 10.1111/j.1365-3083.1989.tb01199.x. View

20.

Sontheimer R . Subacute cutaneous lupus erythematosus: a decade's perspective. Med Clin North Am. 1989; 73(5):1073-90. DOI: 10.1016/s0025-7125(16)30620-4. View