The RNA Binding Protein La Influences Both the Accuracy and the Efficiency of RNA Polymerase III Transcription in Vitro

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1989 Mar 1

PMID 2498086

Citations 93

Authors

E Gottlieb

J A Steitz

Affiliations

Soon will be listed here.

Abstract

The autoantigen La binds the U-rich 3' ends of all nascent RNA polymerase III transcripts. Here, we demonstrate that this abundant nuclear phosphoprotein not only binds these RNAs but appears to be required for their synthesis. HeLa cell extracts immunochemically depleted of La by either patient or mouse monoclonal antibodies lose greater than 99% of their transcription activity on class III genes. The few transcripts synthesized in the absence of La have fewer uridylate residues at their 3' ends than those made in its presence. Reconstitution of La-depleted extracts with biochemically purified HeLa La protein stimulates transcription levels and completely restores transcript length. A model coupling transcription levels to the action of La at the RNA polymerase III termination signal is presented.

Citing Articles

RNA-binding proteins La and HuR cooperatively modulate translation repression of PDCD4 mRNA.

Kumar R, Poria D, Ray P J Biol Chem. 2020; 296:100154.

PMID: 33288677 PMC: 7949077. DOI: 10.1074/jbc.RA120.014894.

Epstein-Barr virus-encoded RNAs (EBERs) complement the loss of Herpesvirus telomerase RNA (vTR) in virus-induced tumor formation.

Kheimar A, Kaufer B Sci Rep. 2018; 8(1):209.

PMID: 29317752 PMC: 5760702. DOI: 10.1038/s41598-017-18638-7.

The RNA binding protein La/SS-B promotes RIG-I-mediated type I and type III IFN responses following Sendai viral infection.

Mahony R, Broadbent L, Maier-Moore J, Power U, Jefferies C Sci Rep. 2017; 7(1):14537.

PMID: 29109527 PMC: 5673980. DOI: 10.1038/s41598-017-15197-9.

Maraia R, Mattijssen S, Cruz-Gallardo I, Conte M Wiley Interdiscip Rev RNA. 2017; 8(6).

PMID: 28782243 PMC: 5647580. DOI: 10.1002/wrna.1430.

La Deletion from Mouse Brain Alters Pre-tRNA Metabolism and Accumulation of Pre-5.8S rRNA, with Neuron Death and Reactive Astrocytosis.

Blewett N, Iben J, Gaidamakov S, Maraia R Mol Cell Biol. 2017; 37(10).

PMID: 28223366 PMC: 5477551. DOI: 10.1128/MCB.00588-16.

References

Rosenberg M, Weissman S, deCrombrugghe B . Termination of transcription in bacteriophage lambda. Heterogeneous, 3'-terminal oligo-adenylate additions and the effects of rho factor. J Biol Chem. 1975; 250(12):4755-64. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Akizuki M, Powers R, HOLMAN H . A soluble acidic protein of the cell nucleus which reacts with serum from patients with systemic lupus erythermatosus and Sjögren's syndrome. J Clin Invest. 1977; 59(2):264-72. PMC: 333356. DOI: 10.1172/JCI108637. View

Brown D, Gurdon J . High-fidelity transcription of 5S DNA injected into Xenopus oocytes. Proc Natl Acad Sci U S A. 1977; 74(5):2064-8. PMC: 431074. DOI: 10.1073/pnas.74.5.2064. View

Provost T . Subsets in systemic lupus erythematosus. J Invest Dermatol. 1979; 72(3):110-3. DOI: 10.1111/1523-1747.ep12530348. View

Weil P, Segall J, Harris B, Ng S, Roeder R . Faithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates. J Biol Chem. 1979; 254(13):6163-73. View

Alspaugh M, Maddison P . Resolution of the identity of certain antigen-antibody systems in systemic lupus erythematosus and Sjögren's syndrome: an interlaboratory collaboration. Arthritis Rheum. 1979; 22(7):796-8. DOI: 10.1002/art.1780220719. View

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

Lerner M, Steitz J . Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1979; 76(11):5495-9. PMC: 411675. DOI: 10.1073/pnas.76.11.5495. View

10.

Engelke D, Ng S, Shastry B, Roeder R . Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes. Cell. 1980; 19(3):717-28. DOI: 10.1016/s0092-8674(80)80048-1. View

11.

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

12.

Peterson R, Doering J, Brown D . Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA. Cell. 1980; 20(1):131-41. DOI: 10.1016/0092-8674(80)90241-x. View

13.

Akusjarvi G, Mathews M, Andersson P, Vennstrom B, Pettersson U . Structure of genes for virus-associated RNAI and RNAII of adenovirus type 2. Proc Natl Acad Sci U S A. 1980; 77(5):2424-8. PMC: 349411. DOI: 10.1073/pnas.77.5.2424. View

14.

Thoen C, Mills K, Hopkins M . Enzyme-linked protein A: an enzyme-linked immunosorbent assay reagent for detecting antibodies in tuberculous exotic animals. Am J Vet Res. 1980; 41(5):833-5. View

15.

Honda B, Roeder R . Association of a 5S gene transcription factor with 5S RNA and altered levels of the factor during cell differentiation. Cell. 1980; 22(1 Pt 1):119-26. DOI: 10.1016/0092-8674(80)90160-9. View

16.

Pelham H, Brown D . A specific transcription factor that can bind either the 5S RNA gene or 5S RNA. Proc Natl Acad Sci U S A. 1980; 77(7):4170-4. PMC: 349792. DOI: 10.1073/pnas.77.7.4170. View

17.

Lerner M, Boyle J, Hardin J, Steitz J . Two novel classes of small ribonucleoproteins detected by antibodies associated with lupus erythematosus. Science. 1981; 211(4480):400-2. DOI: 10.1126/science.6164096. View

18.

Sakonju S, Brown D, Engelke D, Ng S, Shastry B, Roeder R . The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene. Cell. 1981; 23(3):665-9. DOI: 10.1016/0092-8674(81)90429-3. View

19.

Santos T, Zasloff M . Comparative analysis of human chromosomal segments bearing nonallelic dispersed tRNAimet genes. Cell. 1981; 23(3):699-709. DOI: 10.1016/0092-8674(81)90433-5. View

20.

Lerner M, Andrews N, Miller G, Steitz J . Two small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1981; 78(2):805-9. PMC: 319891. DOI: 10.1073/pnas.78.2.805. View