Rapid Method for the Identification of Essential Genes in Staphylococcus Aureus

Overview

Journal Plasmid

Date 1999 Sep 18

PMID 10489332

Citations 10

Authors

M Xia

R D Lunsford

D McDevitt

S Iordanescu

Affiliations

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Abstract

A strategy based on a vector host-dependent for autonomous replication, pSA3182, was utilized both for the rapid screening for Staphylococcus aureus genes essential for cell viability and for the introduction of specific polarity-neutral deletions in nonessential genes. The results obtained support the use of pSA3182 for both purposes.

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