Improved Soft-agar Colony Assay in a Fluid Processing Apparatus

Overview

Journal In Vitro Cell Dev Biol Anim

Publisher Springer

Specialties Biology
Cell Biology

Date 1999 Sep 4

PMID 10475256

Citations 1

Authors

A D Forsman

A R Herpich

S K Chapes

Affiliations

Soon will be listed here.

Abstract

The standard method for quantitating bone marrow precursor cells has been to count the number of colony-forming units that form in semisolid (0.3%) agar. Recently we adapted this assay for use in hardware, the Fluid Processing Apparatus, that is flown in standard payload lockers of the space shuttle. When mouse or rat macrophage colony-forming units were measured with this hardware in ground-based assays, we found significantly more colony growth than that seen in standard plate assays. The improved growth correlates with increased agar thickness but also appears to be due to properties inherent to the Fluid Processing Apparatus. This paper describes an improved method for determining bone marrow macrophage precursor numbers in semisolid agar.

Citing Articles

Understanding macrophage differentiation during space flight: The importance of ground-based experiments before space flight.

Chapes S, Ortega M Recent Pat Space Technol. 2014; 3(1):40-47.

PMID: 24432200 PMC: 3890248. DOI: 10.2174/18776116112029990011.

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