Retroviral Vectors Preloaded with a Viral Receptor-ligand Bridge Protein Are Targeted to Specific Cell Types

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1999 Aug 18

PMID 10449786

Citations 36

Authors

A L Boerger

S Snitkovsky

J A Young

Affiliations

Soon will be listed here.

Abstract

Successful targeting methods represent a major hurdle to the use of retroviral vectors in cell-specific gene-delivery applications. We recently described an approach for retroviral targeting with a retroviral receptor-ligand bridge protein that was bound to the cognate cell-surface ligand receptors before viral challenge. We now report a significant improvement made to this viral targeting method by using a related bridge protein, designated TVB-EGF, comprised of the extracellular domain of the TVB receptor for subgroup B avian leukosis virus fused to epidermal growth factor (EGF). The most important activity of TVB-EGF was that it allowed specific viral entry when preloaded onto virions. Furthermore, virions preloaded with TVB-EGF were thermostable and could be produced directly from virus- packaging cells. These data suggest an approach for targeting retroviral vectors to specific cell types by using virions preloaded with a retroviral receptor-ligand bridge protein and indicate that these types of bridge proteins may be useful reagents for studying the normal mechanism of retroviral entry.

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References

Stern D, Hare D, Cecchini M, Weinberg R . Construction of a novel oncogene based on synthetic sequences encoding epidermal growth factor. Science. 1987; 235(4786):321-4. DOI: 10.1126/science.3492043. View

Jiang A, Chu T, Nocken F, Cichutek K, Dornburg R . Cell-type-specific gene transfer into human cells with retroviral vectors that display single-chain antibodies. J Virol. 1998; 72(12):10148-56. PMC: 110554. DOI: 10.1128/JVI.72.12.10148-10156.1998. View

Glenney Jr J, Chen W, LAZAR C, Walton G, Zokas L, Rosenfeld M . Ligand-induced endocytosis of the EGF receptor is blocked by mutational inactivation and by microinjection of anti-phosphotyrosine antibodies. Cell. 1988; 52(5):675-84. DOI: 10.1016/0092-8674(88)90405-9. View

Roux P, Jeanteur P, Piechaczyk M . A versatile and potentially general approach to the targeting of specific cell types by retroviruses: application to the infection of human cells by means of major histocompatibility complex class I and class II antigens by mouse ecotropic murine.... Proc Natl Acad Sci U S A. 1989; 86(23):9079-83. PMC: 298437. DOI: 10.1073/pnas.86.23.9079. View

Neda H, Wu C, Wu G . Chemical modification of an ecotropic murine leukemia virus results in redirection of its target cell specificity. J Biol Chem. 1991; 266(22):14143-6. View

Landau N, Littman D . Packaging system for rapid production of murine leukemia virus vectors with variable tropism. J Virol. 1992; 66(8):5110-3. PMC: 241381. DOI: 10.1128/JVI.66.8.5110-5113.1992. View

Etienne-Julan M, Roux P, Carillo S, Jeanteur P, Piechaczyk M . The efficiency of cell targeting by recombinant retroviruses depends on the nature of the receptor and the composition of the artificial cell-virus linker. J Gen Virol. 1992; 73 ( Pt 12):3251-5. DOI: 10.1099/0022-1317-73-12-3251. View

Russell S, Hawkins R, Winter G . Retroviral vectors displaying functional antibody fragments. Nucleic Acids Res. 1993; 21(5):1081-5. PMC: 309266. DOI: 10.1093/nar/21.5.1081. View

Valsesia-Wittmann S, Drynda A, Deleage G, Aumailley M, Heard J, Danos O . Modifications in the binding domain of avian retrovirus envelope protein to redirect the host range of retroviral vectors. J Virol. 1994; 68(7):4609-19. PMC: 236388. DOI: 10.1128/JVI.68.7.4609-4619.1994. View

10.

Kasahara N, DOZY A, Kan Y . Tissue-specific targeting of retroviral vectors through ligand-receptor interactions. Science. 1994; 266(5189):1373-6. DOI: 10.1126/science.7973726. View

11.

Wharton S, Calder L, Ruigrok R, Skehel J, Steinhauer D, Wiley D . Electron microscopy of antibody complexes of influenza virus haemagglutinin in the fusion pH conformation. EMBO J. 1995; 14(2):240-6. PMC: 398077. DOI: 10.1002/j.1460-2075.1995.tb06997.x. View

12.

Chu T, Dornburg R . Retroviral vector particles displaying the antigen-binding site of an antibody enable cell-type-specific gene transfer. J Virol. 1995; 69(4):2659-63. PMC: 188950. DOI: 10.1128/JVI.69.4.2659-2663.1995. View

13.

Somia N, Zoppe M, Verma I . Generation of targeted retroviral vectors by using single-chain variable fragment: an approach to in vivo gene delivery. Proc Natl Acad Sci U S A. 1995; 92(16):7570-4. PMC: 41381. DOI: 10.1073/pnas.92.16.7570. View

14.

Cosset F, Morling F, Takeuchi Y, Weiss R, Collins M, Russell S . Retroviral retargeting by envelopes expressing an N-terminal binding domain. J Virol. 1995; 69(10):6314-22. PMC: 189530. DOI: 10.1128/JVI.69.10.6314-6322.1995. View

15.

Hurford Jr R, Dranoff G, Mulligan R, Tepper R . Gene therapy of metastatic cancer by in vivo retroviral gene targeting. Nat Genet. 1995; 10(4):430-5. DOI: 10.1038/ng0895-430. View

16.

Han X, Kasahara N, Kan Y . Ligand-directed retroviral targeting of human breast cancer cells. Proc Natl Acad Sci U S A. 1995; 92(21):9747-51. PMC: 40879. DOI: 10.1073/pnas.92.21.9747. View

17.

Chu T, Martinez I, Sheay W, Dornburg R . Cell targeting with retroviral vector particles containing antibody-envelope fusion proteins. Gene Ther. 1994; 1(5):292-9. View

18.

Matano T, Odawara T, Iwamoto A, Yoshikura H . Targeted infection of a retrovirus bearing a CD4-Env chimera into human cells expressing human immunodeficiency virus type 1. J Gen Virol. 1995; 76 ( Pt 12):3165-9. DOI: 10.1099/0022-1317-76-12-3165. View

19.

Marin M, Noel D, Brockly F, Etienne-Julan M, Russell S, Cosset F . Targeted infection of human cells via major histocompatibility complex class I molecules by Moloney murine leukemia virus-derived viruses displaying single-chain antibody fragment-envelope fusion proteins. J Virol. 1996; 70(5):2957-62. PMC: 190154. DOI: 10.1128/JVI.70.5.2957-2962.1996. View

20.

Valsesia-Wittmann S, Morling F, Nilson B, Takeuchi Y, Russell S, Cosset F . Improvement of retroviral retargeting by using amino acid spacers between an additional binding domain and the N terminus of Moloney murine leukemia virus SU. J Virol. 1996; 70(3):2059-64. PMC: 190040. DOI: 10.1128/JVI.70.3.2059-2064.1996. View