Heterophile Antibodies Segregate in Families and Are Associated with Protection from Type 1 Diabetes

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1999 Jul 8

PMID 10393957

Citations 6

Authors

J X She

T M Ellis

S B Wilson

C H Wasserfall

M Marron

S Reimsneider

S C Kent

D A Hafler

D S Neuberg

A Muir

J L Strominger

M A Atkinson

Affiliations

Soon will be listed here.

Abstract

Markedly elevated levels of serum IL-4 were reported previously in 50% of a small group of type 1 diabetes nonprogessors. To determine the patterns of expression for this phenotype, a larger cohort of 58 families containing type 1 diabetic patients was examined. Analysis of the two-site ELISA assay used to measure serum IL-4 revealed evidence for heterophile antibodies, i.e., nonanalyte substances in serum capable of binding antibodies mutivalently and providing erroneous analyte (e.g., IL-4) quantification. Interestingly, relatives without type 1 diabetes were significantly more likely to have this phenotype than were patients with the disease (P = 0.003). In addition, the trait appears to have clustered within certain families and was associated with the protective MHC allele DQB1*0602 (P = 0.008). These results suggest that heterophile antibodies represent an in vivo trait associated with self-tolerance and nonprogression to diabetes.

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