Structural Analysis of the ADHS Electromorph of Drosophila Melanogaster

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1978 Nov 1

PMID 103098

Citations 23

Authors

T S Fletcher

F J Ayala

D R Thatcher

G K Chambers

Affiliations

Soon will be listed here.

Abstract

Population geneticists have often determined the fitness differences that account for the dynamics of naturally occurring genetic polymorphisms. However, to understand causal aspects of evolutionary processes requires, in addition, investigation of the physiological and molecular structural differences underlying adaptively significant genetic polymorphisms. The characteristics of the alcohol dehydrogenase gene--enzyme system in Drosophila melanogaster make it well suited for this kind of study. Natural populations of this species are polymorphic for two electrophoretically detectable variants, ADHF and ADHS, of the enzyme. Structural studies reported here reveal that the two variants differ by (at least) a single amino acid replacement, threonine in ADHF for lysine in ADHS.

Citing Articles

Sequence variation of alcohol dehydrogenase (Adh) paralogs in cactophilic Drosophila.

Matzkin L, Eanes W Genetics. 2003; 163(1):181-94.

PMID: 12586706 PMC: 1462434. DOI: 10.1093/genetics/163.1.181.

Deletion of a conserved regulatory element in the Drosophila Adh gene leads to increased alcohol dehydrogenase activity but also delays development.

Parsch J, Russell J, Beerman I, Hartl D, Stephan W Genetics. 2000; 156(1):219-27.

PMID: 10978287 PMC: 1461225. DOI: 10.1093/genetics/156.1.219.

A highly conserved sequence in the 3'-untranslated region of the drosophila Adh gene plays a functional role in Adh expression.

Parsch J, Stephan W, Tanda S Genetics. 1999; 151(2):667-74.

PMID: 9927459 PMC: 1460503. DOI: 10.1093/genetics/151.2.667.

Substrate and inhibitor specificities of the thermostable alcohol dehydrogenase allozymes ADH-71k and ADH-FCh.D. of Drosophila melanogaster.

Eisses K, Davies S, Chambers G Biochem Genet. 1994; 32(3-4):91-103.

PMID: 7980388 DOI: 10.1007/BF00554418.

Genetic polymorphism: from electrophoresis to DNA sequences.

Ayala F Experientia. 1983; 39(8):813-23.

PMID: 6873231 DOI: 10.1007/BF01990397.

References

ODonnell J, Gerace L, Leister F, Sofer W . Chemical selection of mutants that affect alcohol dehydrogenase in Drosophila. II. Use of 1-pentyne-3-ol. Genetics. 1975; 79(1):73-83. PMC: 1213261. DOI: 10.1093/genetics/79.1.73. View

McDonald J, Ayala F . Genetic and biochemical basis of enzyme activity variation in natural populations. I. Alcohol dehydrogenase in Drosophila melanogaster. Genetics. 1978; 89(2):371-88. PMC: 1213843. DOI: 10.1093/genetics/89.2.371. View

Crestfield A, Moore S, STEIN W . The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins. J Biol Chem. 1963; 238:622-7. View

Davis B . DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964; 121:404-27. DOI: 10.1111/j.1749-6632.1964.tb14213.x. View

Mendez E, Lai C . Regeneration of amino acids from thiazolinones formed in the Edman degradation. Anal Biochem. 1975; 68(1):47-53. DOI: 10.1016/0003-2697(75)90677-6. View

Thatcher D . Enzyme instability and proteolysis during the purification of an alcohol dehydrogenase from Drosophila melanogaster. Biochem J. 1977; 163(2):317-23. PMC: 1164699. DOI: 10.1042/bj1630317. View

Thatcher D, Camfield R . Chemical basis of the electrophoretic variation between two naturally occurring alcohol dehydrogenase alloenzymes from Drosophila melanogaster. Biochem Soc Trans. 1977; 5(1):271-2. DOI: 10.1042/bst0050271. View

McDonald J, Chambers G, David J, Ayala F . Adaptive response due to changes in gene regulation: a study with Drosophila. Proc Natl Acad Sci U S A. 1977; 74(10):4562-6. PMC: 431986. DOI: 10.1073/pnas.74.10.4562. View

Thompson Jr J, Ashburner M, Woodruff R . Presumptive control mutation for alcohol dehydrogenase in Drosophila melanogaster. Nature. 1977; 270(5635):363. DOI: 10.1038/270363a0. View

10.

Day T, Hillier P, Clarke B . Properties of genetically polymorphic isozymes of alcohol dehydrogenase in Drosophila melanogaster. Biochem Genet. 1974; 11(2):141-53. DOI: 10.1007/BF00485770. View

11.

Day T, Hillier P, Clarke B . The relative quantities and catalytic activities of enzymes produced by alleles at the alcohol dehydrogenase locus in Drosophila melanogaster. Biochem Genet. 1974; 11(2):155-65. DOI: 10.1007/BF00485771. View

12.

McKenzie J, Parsons P . Microdifferentiation in a natural population of Drosophila melanogaster to alcohol in the environment. Genetics. 1974; 77(2):385-94. PMC: 1213135. DOI: 10.1093/genetics/77.2.385. View

13.

Day T, Needham L . Properties of alcohol dehydrogenase isozymes in a strain of Drosophila melanogaster homozygous for the Adh-slow allele. Biochem Genet. 1974; 11(2):167-75. DOI: 10.1007/BF00485772. View

14.

MERRITT A, Rivas M, Ward J . Evidence for close linkage of human amylase loci. Nat New Biol. 1972; 239(95):243-4. DOI: 10.1038/newbio239243a0. View

15.

Kulbe K . Micropolyamide thin-layer chromatography of phenylthiohydantoin amino acids (PTH) at subnanomolar level. A rapid microtechnique for simultaneous multisample identification after automated Edman degradations. Anal Biochem. 1974; 59(2):564-73. DOI: 10.1016/0003-2697(74)90310-8. View

16.

Ayala F, Powell J, Tracey M, Mourao C, Perez-Salas S . Enzyme variability in the Drosophila willistoni group. IV. Genic variation in natural populations of Drosophila willistoni. Genetics. 1972; 70(1):113-39. PMC: 1212716. DOI: 10.1093/genetics/70.1.113. View

17.

Pisano J, Bronzert T, BREWER Jr H . Advances in the gas chromatographic analysis of amino acid phenyl- and methylthiohydantoins. Anal Biochem. 1972; 45(1):43-59. DOI: 10.1016/0003-2697(72)90006-1. View

18.

Grell E, Jacobson K, Murphy J . Alterations of genetics material for analysis of alcohol dehydrogenase isozymes of Drosophila melanogaster. Ann N Y Acad Sci. 1968; 151(1):441-55. DOI: 10.1111/j.1749-6632.1968.tb11907.x. View

19.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

20.

Briscoe D, Robertson A, Malpica J . Dominance at Adh locus in response of adult Drosophila melanogaster to environmental alcohol. Nature. 1975; 255(5504):148-9. DOI: 10.1038/255148a0. View