Alpha 2.0
Login Register
» Articles » PMID: 10233060

COVOL: an Interactive Program for Evaluating Second Virial Coefficients from the Triaxial Shape or Dimensions of Rigid Macromolecules

Overview
Journal Biophys J
Publisher Cell Press
Specialty Biophysics
Date 1999 May 8
PMID 10233060
Citations 19
Authors
S E Harding
J C Horton
S Jones
J M Thornton
D J Winzor
Affiliations
Soon will be listed here.
Abstract

An interactive program is described for calculating the second virial coefficient contribution to the thermodynamic nonideality of solutions of rigid macromolecules based on their triaxial dimensions. The FORTRAN-77 program, available in precompiled form for the PC, is based on theory for the covolume of triaxial ellipsoid particles [Rallison, J. M., and S.E Harding. (1985). J. Colloid Interface Sci. 103:284-289]. This covolume has the potential to provide a magnitude for the second virial coefficient of macromolecules bearing no net charge. Allowance for a charge-charge contribution is made via an expression based on Debye-Hückel theory and uniform distribution of the net charge over the surface of a sphere with dimensions governed by the Stokes radius of the macromolecule. Ovalbumin, ribonuclease A, and hemoglobin are used as model systems to illustrate application of the COVOL routine.

Citing Articles

Experimental support for reclassification of the light scattering second virial coefficient from macromolecular solutions as a hydrodynamic parameter.

Winzor D, Dinu V, Scott D, Harding S Eur Biophys J. 2023; 52(4-5):343-352.

PMID: 37460663 PMC: 10444693. DOI: 10.1007/s00249-023-01665-w.

Retrospective rationalization of disparities between the concentration dependence of diffusion coefficients obtained by boundary spreading and dynamic light scattering.

Winzor D, Dinu V, Scott D, Harding S Eur Biophys J. 2023; 52(4-5):333-342.

PMID: 37414903 PMC: 10444695. DOI: 10.1007/s00249-023-01664-x.

"meet the editors series"-a profile of Steve Harding's career in macromolecular hydrodynamics.

Harding S Biophys Rev. 2022; 14(3):605-610.

PMID: 35791390 PMC: 9250574. DOI: 10.1007/s12551-022-00963-5.

Analytical Ultracentrifugation as a Matrix-Free Probe for the Study of Kinase Related Cellular and Bacterial Membrane Proteins and Glycans.

Harding S Molecules. 2021; 26(19).

PMID: 34641622 PMC: 8512968. DOI: 10.3390/molecules26196080.

Quantifying the concentration dependence of sedimentation coefficients for globular macromolecules: a continuing age-old problem.

Winzor D, Dinu V, Scott D, Harding S Biophys Rev. 2021; 13(2):273-288.

PMID: 33936319 PMC: 8046895. DOI: 10.1007/s12551-021-00793-x.

References
1.
Baghurst P, Nichol L, OGSTON A, Winzor D . Quantitative interpretation of concentration-dependent migration in gel chromatography of reversibly polymerizing solutes. Biochem J. 1975; 147(3):575-83. PMC: 1165485. DOI: 10.1042/bj1470575. View
2.
Ross P, Minton A . The effect of non-aggregating proteins upon the gelation of sickle cell hemoglobin: model calculations and data analysis. Biochem Biophys Res Commun. 1979; 88(4):1308-14. DOI: 10.1016/0006-291x(79)91123-9. View
3.
Wills P, Nichol L, Siezen R . The indefinite self-association of lysozyme: consideration of composition-dependent activity coefficients. Biophys Chem. 1980; 11(1):71-82. DOI: 10.1016/0301-4622(80)85009-5. View
4.
FERMI G, Perutz M, Shaanan B, Fourme R . The crystal structure of human deoxyhaemoglobin at 1.74 A resolution. J Mol Biol. 1984; 175(2):159-74. DOI: 10.1016/0022-2836(84)90472-8. View
5.
Shearwin K, Winzor D . Thermodynamic nonideality in macromolecular solutions. Evaluation of parameters for the prediction of covolume effects. Eur J Biochem. 1990; 190(3):523-9. DOI: 10.1111/j.1432-1033.1990.tb15605.x. View