Expression, Purification and Preliminary X-ray Crystallographic Analysis of PsaA, a Putative Metal-transporter Protein of Streptococcus Pneumoniae

Overview

Journal Acta Crystallogr D Biol Crystallogr

Specialty Chemistry

Date 1999 Mar 25

PMID 10089539

Citations 14

Authors

P A Pilling

M C Lawrence

A M Berry

A D Ogunniyi

R A Lock

J C Paton

Affiliations

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Abstract

The putative metal-transporter protein PsaA of Streptococcus pneumoniae is of potential interest both as a vaccine and also as a drug target. The overexpression of the protein in E. coli, and its subsequent purification and crystallization are described. The crystals are rectangular rods and diffract to beyond 2.7 A resolution. The crystal space group is P212121 with unit-cell dimensions a = 59.9, b = 66.5 and c = 69.9 A.

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