Oxidative Resistance of Na/K-ATPase

Overview

Journal Cell Mol Neurobiol

Publisher Springer

Specialties Cell Biology
Molecular Biology
Neurology

Date 1999 Mar 18

PMID 10079972

Citations 17

Authors

E G Kurella

O V Tyulina

A A Boldyrev

Affiliations

Soon will be listed here.

Abstract

1. Oxidative modification of Na/K-ATPase from brain and kidney has been studied. Brain enzyme has been found to be more sensitive than kidney enzyme to inhibition by both H2O2 and NaOCl. 2. The inhibition of Na/K-ATPase correlates well with the decrease in a number of SH groups, suggesting that the latter belong mainly to ATPase protein and are essential for the enzyme activity. We suggest that the differences in the number, location, and accessibility of SH groups in Na/K-ATPase isozymes predict their oxidative stability. 3. The hydrophilic natural antioxidant carnosine, the hydrophobic natural antioxidant alpha-tocopherol, and the synthetic antioxidant ionol as well as the ferrous ion chelating agent deferoxamine were found to protect Na/K-ATPase from oxidation by different concentrations of H2O2. The data suggest that these antioxidants are effective due to their ability to neutralize or to prevent formation of hydroxyl radicals.

Citing Articles

Hypoxia and HIF-1α Regulate the Activity and Expression of Na,K-ATPase Subunits in H9c2 Cardiomyoblasts.

Gurler B, Gencay G, Baloglu E Curr Issues Mol Biol. 2023; 45(10):8277-8288.

PMID: 37886965 PMC: 10605391. DOI: 10.3390/cimb45100522.

Hypoxic Stress-Dependent Regulation of Na,K-ATPase in Ischemic Heart Disease.

Baloglu E Int J Mol Sci. 2023; 24(9).

PMID: 37175562 PMC: 10177966. DOI: 10.3390/ijms24097855.

Involvement of plasma membrane H-ATPase in diamide-induced extracellular alkalization by roots from pea seedlings.

Lapshin N, Piotrovskii M, Trofimova M Planta. 2021; 253(1):10.

PMID: 33389194 DOI: 10.1007/s00425-020-03532-w.

Hypoxia attenuate ionic transport in the isolated gill epithelium of Carcinus maenas.

Lucu C J Comp Physiol B. 2020; 190(4):391-401.

PMID: 32333115 DOI: 10.1007/s00360-020-01277-2.

The Redox-Sensitive Na/K-ATPase Signaling in Uremic Cardiomyopathy.

Liu J, Nie Y, Chaudhry M, Bai F, Chuang J, Sodhi K Int J Mol Sci. 2020; 21(4).

PMID: 32069992 PMC: 7072896. DOI: 10.3390/ijms21041256.

References

Xie Z, Wang Y, Periyasamy S, Blanco G, Huang W, Askari A . Different oxidant sensitivities of the alpha 1 and alpha 2 isoforms of Na+/K(+)-ATPase expressed in baculovirus-infected insect cells. Biochem Biophys Res Commun. 1995; 207(1):155-9. DOI: 10.1006/bbrc.1995.1166. View

Vasilets L, Schwarz W . Structure-function relationships of cation binding in the Na+/K(+)-ATPase. Biochim Biophys Acta. 1993; 1154(2):201-22. DOI: 10.1016/0304-4157(93)90012-d. View

Ellman G . Tissue sulfhydryl groups. Arch Biochem Biophys. 1959; 82(1):70-7. DOI: 10.1016/0003-9861(59)90090-6. View

Kong Y, Lesnefsky E, Ye J, Horwitz L . Prevention of lipid peroxidation does not prevent oxidant-induced myocardial contractile dysfunction. Am J Physiol. 1994; 267(6 Pt 2):H2371-7. DOI: 10.1152/ajpheart.1994.267.6.H2371. View

Boldyrev A, Bulygina E, Volynskaia E, Kurella E, Tiulina O . [The effect of hydrogen peroxide and hypochlorite on brain Na,K-ATPase activity]. Biokhimiia. 1995; 60(10):1688-96. View

KAKO K, Kato M, Matsuoka T, Mustapha A . Depression of membrane-bound Na+-K+-ATPase activity induced by free radicals and by ischemia of kidney. Am J Physiol. 1988; 254(2 Pt 1):C330-7. DOI: 10.1152/ajpcell.1988.254.2.C330. View

Pedemonte C, Kaplan J . Chemical modification as an approach to elucidation of sodium pump structure-function relations. Am J Physiol. 1990; 258(1 Pt 1):C1-23. DOI: 10.1152/ajpcell.1990.258.1.C1. View

Boldyrev A, Abe H, Stvolinsky S, Tyulina O . Effects of carnosine and related compounds on generation of free oxygen species: a comparative study. Comp Biochem Physiol B Biochem Mol Biol. 1995; 112(3):481-5. DOI: 10.1016/0305-0491(95)00084-4. View

Lees G . Inhibition of sodium-potassium-ATPase: a potentially ubiquitous mechanism contributing to central nervous system neuropathology. Brain Res Brain Res Rev. 1991; 16(3):283-300. DOI: 10.1016/0165-0173(91)90011-v. View

10.

NORBY J . Coupled assay of Na+,K+-ATPase activity. Methods Enzymol. 1988; 156:116-9. DOI: 10.1016/0076-6879(88)56014-7. View

11.

Thomas C, Reed D . Radical-induced inactivation of kidney Na+,K(+)-ATPase: sensitivity to membrane lipid peroxidation and the protective effect of vitamin E. Arch Biochem Biophys. 1990; 281(1):96-105. DOI: 10.1016/0003-9861(90)90418-x. View

12.

Olanow C . A radical hypothesis for neurodegeneration. Trends Neurosci. 1993; 16(11):439-44. DOI: 10.1016/0166-2236(93)90070-3. View

13.

Boldyrev A . Does carnosine possess direct antioxidant activity?. Int J Biochem. 1993; 25(8):1101-7. DOI: 10.1016/0020-711x(93)90587-5. View

14.

Rathbun W, Betlach M . Estimation of enzymically produced orthophosphate in the presence of cysteine and adenosine triphosphate. Anal Biochem. 1969; 28(1):436-45. DOI: 10.1016/0003-2697(69)90198-5. View

15.

Kukreja R, Weaver A, Hess M . Sarcolemmal Na(+)-K(+)-ATPase: inactivation by neutrophil-derived free radicals and oxidants. Am J Physiol. 1990; 259(5 Pt 2):H1330-6. DOI: 10.1152/ajpheart.1990.259.5.H1330. View

16.

Kurella E, Osipov A, Goldman R, Boldyrev A, Kagan V . Inhibition of Na+/K(+)-ATPase by phenoxyl radicals of etoposide (VP-16): role of sulfhydryls oxidation. Biochim Biophys Acta. 1995; 1232(1-2):52-8. DOI: 10.1016/0005-2728(95)00115-0. View

17.

Boldyrev A, Stvolinsky S, Tyulina O, Koshelev V, Hori N, Carpenter D . Biochemical and physiological evidence that carnosine is an endogenous neuroprotector against free radicals. Cell Mol Neurobiol. 1997; 17(2):259-71. PMC: 11560219. DOI: 10.1023/a:1026374114314. View

18.

Boldyrev A, Bulygina E, Kramarenko G, Vanin A . Effect of nitroso compounds on Na/K-ATPase. Biochim Biophys Acta. 1997; 1321(3):243-51. DOI: 10.1016/s0005-2728(97)00053-4. View

19.

Klodos I, Ottolenghi P . Large-scale preparation of Na, K-ATPase from ox brain. Anal Biochem. 1975; 67(2):397-403. DOI: 10.1016/0003-2697(75)90311-5. View

20.

Zolotarjova N, Ho C, Mellgren R, Askari A, Huang W . Different sensitivities of native and oxidized forms of Na+/K(+)-ATPase to intracellular proteinases. Biochim Biophys Acta. 1994; 1192(1):125-31. DOI: 10.1016/0005-2736(94)90152-x. View