Heat Resistance of Native and Demineralized Spores of Bacillus Subtilis Sporulated at Different Temperatures

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1999 Mar 2

PMID 10049900

Authors

A Palop

F J Sala

S Condon

Affiliations

Soon will be listed here.

Abstract

Demineralization reduced heat resistance of B. subtilis spores, but the pattern and magnitude of the reduction depended on sporulation temperature and on heating menstruum pH. The differences in heat resistance of native spores caused by sporulation temperature almost disappeared after demineralization. Demineralized spores were still susceptible to the heat-sensitizing effect of acidic pH.

References

Lechowich R, Ordal Z . The influence of the sporulation temperature on the heat resistance and chemical composition of bacterial spores. Can J Microbiol. 1962; 8:287-95. DOI: 10.1139/m62-040. View

Abraham G, Debray E, Candau Y, Piar G . Mathematical Model of Thermal Destruction of Bacillus stearothermophilus Spores. Appl Environ Microbiol. 1990; 56(10):3073-80. PMC: 184901. DOI: 10.1128/aem.56.10.3073-3080.1990. View

RODE L, FOSTER J . Quantitative aspects of exchangeable calcium in spores of Bacillus megaterium. J Bacteriol. 1966; 91(4):1589-93. PMC: 316082. DOI: 10.1128/jb.91.4.1589-1593.1966. View

Ando Y, Tsuzuki T . Mechanism of chemical manipulation of the heat resistance of Clostridium perfringens spores. J Appl Bacteriol. 1983; 54(2):197-202. DOI: 10.1111/j.1365-2672.1983.tb02607.x. View

ALDERTON G, SNELL N . Base exchange and heat resistance in bacterial spores. Biochem Biophys Res Commun. 1963; 10:139-43. DOI: 10.1016/0006-291x(63)90039-1. View

Beaman T, Gerhardt P . Heat resistance of bacterial spores correlated with protoplast dehydration, mineralization, and thermal adaptation. Appl Environ Microbiol. 1986; 52(6):1242-6. PMC: 239215. DOI: 10.1128/aem.52.6.1242-1246.1986. View

SHULL J, CARGO G, Ernst R . KINETICS OF HEAT ACTIVATION AND OF THERMAL DEATH OF BACTERIAL SPORES. Appl Microbiol. 1963; 11:485-7. PMC: 1058035. DOI: 10.1128/am.11.6.485-487.1963. View

ALDERTON G, Chen J, Ito K . Heat resistance of the chemical resistance forms of Clostridium botulinum 62A spores over the water activity range 0 to 0.9. Appl Environ Microbiol. 1980; 40(3):511-5. PMC: 291614. DOI: 10.1128/aem.40.3.511-515.1980. View

Heredia N, Garcia G, Luevanos R, Labbe R, Garcia-Alvarado J . Elevation of the Heat Resistance of Vegetative Cells and Spores of Clostridium perfringens Type A by Sublethal Heat Shock. J Food Prot. 2019; 60(8):998-1000. DOI: 10.4315/0362-028X-60.8.998. View

10.

Condon S, Bayarte M, Sala F . Influence of the sporulation temperature upon the heat resistance of Bacillus subtilis. J Appl Bacteriol. 1992; 73(3):251-6. DOI: 10.1111/j.1365-2672.1992.tb02985.x. View

11.

Gould G, Dring G . Heat resistance of bacterial endospores and concept of an expanded osmoregulatory cortex. Nature. 1975; 258(5534):402-5. DOI: 10.1038/258402a0. View

12.

ALDERTON G, Ito K, Chen J . Chemical manipulation of the heat resistance of Clostridium botulinum spores. Appl Environ Microbiol. 1976; 31(4):492-8. PMC: 169810. DOI: 10.1128/aem.31.4.492-498.1976. View

13.

Bender G, Marquis R . Spore heat resistance and specific mineralization. Appl Environ Microbiol. 1985; 50(6):1414-21. PMC: 238773. DOI: 10.1128/aem.50.6.1414-1421.1985. View

14.

SLEPECKY R, FOSTER J . Alterations in metal content of spores of Bacillus megaterium and the effect on some spore properties. J Bacteriol. 1959; 78(1):117-23. PMC: 290492. DOI: 10.1128/jb.78.1.117-123.1959. View

15.

Stewart M, Somlyo A, Somlyo A, Shuman H, Lindsay J, Murrell W . Distribution of calcium and other elements in cryosectioned Bacillus cereus T spores, determined by high-resolution scanning electron probe x-ray microanalysis. J Bacteriol. 1980; 143(1):481-91. PMC: 294274. DOI: 10.1128/jb.143.1.481-491.1980. View

16.

Sala F, Ibarz P, Palop A, Raso J, Condon S . Sporulation Temperature and Heat Resistance of Bacillus subtilis at Different pH Values. J Food Prot. 2019; 58(3):239-243. DOI: 10.4315/0362-028X-58.3.239. View