The Exocyst is an Effector for Sec4p, Targeting Secretory Vesicles to Sites of Exocytosis

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1999 Feb 18

PMID 10022848

Citations 288

Authors

W Guo

D Roth

P Novick

Affiliations

Soon will be listed here.

Abstract

Polarized secretion requires proper targeting of secretory vesicles to specific sites on the plasma membrane. Here we report that the exocyst complex plays a key role in vesicle targeting. Sec15p, an exocyst component, can associate with secretory vesicles and interact specifically with the rab GTPase, Sec4p, in its GTP-bound form. A chain of protein-protein interactions leads from Sec4p and Sec15p on the vesicle, through various subunits of the exocyst, to Sec3p, which marks the sites of exocytosis on the plasma membrane. Sec4p may control the assembly of the exocyst. The exocyst may therefore function as a rab effector system for targeted secretion.

Citing Articles

RAB-10 cooperates with EHBP-1 to capture vesicular carriers during post-Golgi exocytic trafficking.

Liu S, Wei J, Zhong L, Hai S, Song S, Xie C J Cell Biol. 2025; 224(4).

PMID: 39982707 PMC: 11844438. DOI: 10.1083/jcb.202410003.

The exocyst complex controls multiple events in the pathway of regulated exocytosis.

Suarez Freire S, Perez-Pandolfo S, Fresco S, Valinoti J, Sorianello E, Wappner P Elife. 2024; 12.

PMID: 39585321 PMC: 11588341. DOI: 10.7554/eLife.92404.

Distinct trafficking routes of polarized and non-polarized membrane cargoes in .

Sagia G, Georgiou X, Chamilos G, Diallinas G, Dimou S Elife. 2024; 13.

PMID: 39431919 PMC: 11578586. DOI: 10.7554/eLife.103355.

The exocyst in context.

Meek S, Hernandez A, Oliva B, Gallego O Biochem Soc Trans. 2024; 52(5):2113-2122.

PMID: 39377315 PMC: 11555703. DOI: 10.1042/BST20231401.

Exocyst stimulates multiple steps of exocytic SNARE complex assembly and vesicle fusion.

Lee C, Lepore D, Lee S, Kim T, Buwa N, Lee J Nat Struct Mol Biol. 2024; 32(1):150-160.

PMID: 39242980 DOI: 10.1038/s41594-024-01388-2.

References

Brennwald P, Kearns B, Champion K, Keranen S, Bankaitis V, Novick P . Sec9 is a SNAP-25-like component of a yeast SNARE complex that may be the effector of Sec4 function in exocytosis. Cell. 1994; 79(2):245-58. DOI: 10.1016/0092-8674(94)90194-5. View

Sacher M, Jiang Y, BARROWMAN J, Scarpa A, Burston J, Zhang L . TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion. EMBO J. 1998; 17(9):2494-503. PMC: 1170591. DOI: 10.1093/emboj/17.9.2494. View

Govindan B, Bowser R, Novick P . The role of Myo2, a yeast class V myosin, in vesicular transport. J Cell Biol. 1995; 128(6):1055-68. PMC: 2120422. DOI: 10.1083/jcb.128.6.1055. View

Terbush D, Novick P . Sec6, Sec8, and Sec15 are components of a multisubunit complex which localizes to small bud tips in Saccharomyces cerevisiae. J Cell Biol. 1995; 130(2):299-312. PMC: 2199927. DOI: 10.1083/jcb.130.2.299. View

Ting A, Hazuka C, Hsu S, Kirk M, Bean A, Scheller R . rSec6 and rSec8, mammalian homologs of yeast proteins essential for secretion. Proc Natl Acad Sci U S A. 1995; 92(21):9613-7. PMC: 40852. DOI: 10.1073/pnas.92.21.9613. View

Stenmark H, Vitale G, Ullrich O, Zerial M . Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion. Cell. 1995; 83(3):423-32. DOI: 10.1016/0092-8674(95)90120-5. View

Schneider B, Seufert W, Steiner B, Yang Q, Futcher A . Use of polymerase chain reaction epitope tagging for protein tagging in Saccharomyces cerevisiae. Yeast. 1995; 11(13):1265-74. DOI: 10.1002/yea.320111306. View

Sapperstein S, Lupashin V, Schmitt H, Waters M . Assembly of the ER to Golgi SNARE complex requires Uso1p. J Cell Biol. 1996; 132(5):755-67. PMC: 2120746. DOI: 10.1083/jcb.132.5.755. View

Ren M, Zeng J, Rosenfeld M, Adesnik M, Sabatini D . In its active form, the GTP-binding protein rab8 interacts with a stress-activated protein kinase. Proc Natl Acad Sci U S A. 1996; 93(10):5151-5. PMC: 39423. DOI: 10.1073/pnas.93.10.5151. View

10.

Pfeffer S . Transport vesicle docking: SNAREs and associates. Annu Rev Cell Dev Biol. 1996; 12:441-61. DOI: 10.1146/annurev.cellbio.12.1.441. View

11.

Terbush D, Maurice T, Roth D, Novick P . The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. EMBO J. 1996; 15(23):6483-94. PMC: 452473. View

12.

Goud B, Salminen A, Walworth N, Novick P . A GTP-binding protein required for secretion rapidly associates with secretory vesicles and the plasma membrane in yeast. Cell. 1988; 53(5):753-68. DOI: 10.1016/0092-8674(88)90093-1. View

13.

Cunningham K, Wickner W . Yeast KEX2 protease and mannosyltransferase I are localized to distinct compartments of the secretory pathway. Yeast. 1989; 5(1):25-33. DOI: 10.1002/yea.320050105. View

14.

Walworth N, Goud B, Kabcenell A, Novick P . Mutational analysis of SEC4 suggests a cyclical mechanism for the regulation of vesicular traffic. EMBO J. 1989; 8(6):1685-93. PMC: 401010. DOI: 10.1002/j.1460-2075.1989.tb03560.x. View

15.

Salminen A, Novick P . The Sec15 protein responds to the function of the GTP binding protein, Sec4, to control vesicular traffic in yeast. J Cell Biol. 1989; 109(3):1023-36. PMC: 2115742. DOI: 10.1083/jcb.109.3.1023. View

16.

Sasaki T, Kikuchi A, Araki S, Hata Y, Isomura M, Kuroda S . Purification and characterization from bovine brain cytosol of a protein that inhibits the dissociation of GDP from and the subsequent binding of GTP to smg p25A, a ras p21-like GTP-binding protein. J Biol Chem. 1990; 265(4):2333-7. View

17.

REDDING K, Holcomb C, Fuller R . Immunolocalization of Kex2 protease identifies a putative late Golgi compartment in the yeast Saccharomyces cerevisiae. J Cell Biol. 1991; 113(3):527-38. PMC: 2288974. DOI: 10.1083/jcb.113.3.527. View

18.

Chien C, Bartel P, Sternglanz R, Fields S . The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proc Natl Acad Sci U S A. 1991; 88(21):9578-82. PMC: 52761. DOI: 10.1073/pnas.88.21.9578. View

19.

Grindstaff K, Yeaman C, Anandasabapathy N, Hsu S, Rodriguez-Boulan E, Scheller R . Sec6/8 complex is recruited to cell-cell contacts and specifies transport vesicle delivery to the basal-lateral membrane in epithelial cells. Cell. 1998; 93(5):731-40. DOI: 10.1016/s0092-8674(00)81435-x. View

20.

Roth D, Guo W, Novick P . Dominant negative alleles of SEC10 reveal distinct domains involved in secretion and morphogenesis in yeast. Mol Biol Cell. 1998; 9(7):1725-39. PMC: 25411. DOI: 10.1091/mbc.9.7.1725. View