Wendy F Rothwell
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Explore the profile of Wendy F Rothwell including associated specialties, affiliations and a list of published articles.
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Recent Articles
1.
Rothwell W, Sullivan W
CSH Protoc
. 2011 Mar;
2007:pdb.prot4828.
PMID: 21357168
INTRODUCTIONFixed Drosophila embryos stored in methanol must be rehydrated before staining. Rehydration methods differ slightly for embryos fixed in methanol versus embryos fixed in formaldehyde; techniques for both are presented....
2.
Rothwell W, Sullivan W
CSH Protoc
. 2011 Mar;
2007:pdb.prot4827.
PMID: 21357167
INTRODUCTIONA number of techniques are available for fixation of Drosophila embryos, each with their own advantages and limitations. The technique appropriate for a specific study will depend on a number...
3.
Rothwell W, Sullivan W
CSH Protoc
. 2011 Mar;
2007:pdb.prot4826.
PMID: 21357166
INTRODUCTIONEarly Drosophila embryos are particularly amenable to cellular analysis. However, they are protected by an outer chorion layer, as well as an impermeable and opaque vitelline membrane. Consequently, preparation of...
4.
Rothwell W, Sullivan W
CSH Protoc
. 2011 Mar;
2007:pdb.prot4825.
PMID: 21357165
INTRODUCTIONA number of factors make the early Drosophila embryo particularly amenable to cellular analysis. First, large numbers of specifically staged embryos are easily collected from normal and mutant stocks. Also,...
5.
Rothwell W, Sullivan W
CSH Protoc
. 2011 Mar;
2007:pdb.prot4834.
PMID: 21356945
INTRODUCTIONDrosophila embryos can be stained with specific fluorescent probes or antibodies through either direct or indirect immunofluorescence. In particular, several effective probes exist for visualizing DNA. 4',6-diamidino-2-phenylindole (DAPI) is a...
6.
Rothwell W, Sullivan W
CSH Protoc
. 2011 Mar;
2007:pdb.prot4833.
PMID: 21356944
INTRODUCTIONDrosophila embryos can be stained with specific fluorescent probes or antibodies through either direct or indirect immunofluorescence. In particular, several effective probes exist for visualizing DNA. Propidium iodide (PI), a...
7.
Rothwell W, Sullivan W
CSH Protoc
. 2011 Mar;
2007:pdb.ip44.
PMID: 21356941
INTRODUCTIONA variety of probes and stains are available for labeling specific structures in Drosophila embryos. This article lists some commercially available reagents used for visualizing specific structures.