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Tsutomu Arakawa

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Articles 226
Citations 2034
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Recent Articles
1.
Arakawa T, Ejima D, Tomioka Y, Sakuma C, Akuta T
Protein Expr Purif . 2025 Feb; 230:106690. PMID: 39978707
We review here several niche downstream purification processes that are not covered by other articles in this special issue. The first is the use of activated carbon to capture contaminants...
2.
Akuta T, Tomioka Y, Ura T, Nakagawa M, Arakawa T
Protein J . 2025 Jan; 44(1):79-87. PMID: 39755991
The Ferguson plot is a simple method for determining the molecular weight of native proteins and their complexes. In this study, we tested the validity of the Ferguson plot based...
3.
Akuta T, Ura T, Oikawa T, Tomioka Y, Eguchi A, Arakawa T
Biophys Chem . 2024 Aug; 314:107316. PMID: 39168056
We have studied binding properties of three detergents, i.e., sodium dodecyl sulfate (SDS), Sarkosyl and sodium lauroyl glutamate (SLG), to model proteins based on their effects on electrophoretic mobilities of...
4.
Arakawa T, Tomioka Y, Akuta T, Shiraki K
Biophys Chem . 2024 Jun; 312:107282. PMID: 38944944
Protein aggregation is a major hurdle in developing biopharmaceuticals, in particular protein formulation area, but plays a pivotal role in food products. Co-solvents are used to suppress protein aggregation in...
5.
Tomioka Y, Akuta T, Tokunaga M, Arakawa T
Biophys Chem . 2024 Feb; 307:107200. PMID: 38367540
In this study, we conducted Ferguson plot analyses using both agarose and polyacrylamide gels in native electrophoresis and SDS-PAGE. The results revealed intriguing differences in the behavior of bovine serum...
6.
Arakawa T, Niikura T, Kita Y, Akuta T
Curr Issues Mol Biol . 2024 Jan; 46(1):621-633. PMID: 38248342
In this study, we review the properties of three anionic detergents, sodium dodecyl sulfate (SDS), Sarkosyl, and sodium lauroylglutamate (SLG), as they play a critical role in molecular biology research....
7.
Arakawa T, Nakagawa M, Sakuma C, Tomioka Y, Kurosawa Y, Ejima D, et al.
Eur Biophys J . 2023 Dec; 53(1-2):1-13. PMID: 38160206
Gel electrophoresis, a transport technology, is one of the most widely used experimental methods in biochemical and pharmaceutical research and development. Transport technologies are used to determine hydrodynamic or electrophoretic...
8.
Arakawa T, Akuta T
Antibodies (Basel) . 2023 Oct; 12(4). PMID: 37873861
The poly-reactivity of antibodies is defined as their binding to specific antigens as well as to related proteins and also to unrelated targets. Poly-reactivity can occur in individual molecules of...
9.
Tomioka Y, Nagatoishi S, Nakagawa M, Tsumoto K, Arakawa T, Akuta T
Biophys Chem . 2023 Aug; 301:107095. PMID: 37611350
Ferguson plot was used to characterize the multiple intermediate species of bovine serum albumin (BSA) upon thermal unfolding. Differential scanning calorimetry showed an irreversible melting of BSA in Tris-HCl and...
10.
Nakagawa M, Tomioka Y, Sakuma C, Kurosawa Y, Shibata T, Arakawa T, et al.
Electrophoresis . 2023 Jun; 44(17-18):1446-1460. PMID: 37294166
A new protocol for conducting two-dimensional (2D) electrophoresis was developed by combining the recently developed agarose native gel electrophoresis with either vertical sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE)...