Todd A Duncombe
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Explore the profile of Todd A Duncombe including associated specialties, affiliations and a list of published articles.
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Articles
15
Citations
374
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Recent Articles
1.
Duncombe T, Ponti A, Seebeck F, Dittrich P
Anal Chem
. 2021 Sep;
93(38):13008-13013.
PMID: 34533299
We introduce the UV-vis spectra-activated droplet sorter (UVADS) for high-throughput label-free chemical identification and enzyme screening. In contrast to previous absorbance-based droplet sorters that relied on single-wavelength absorbance in the...
2.
Bachler S, Haidas D, Ort M, Duncombe T, Dittrich P
Commun Biol
. 2020 Dec;
3(1):769.
PMID: 33318607
In the field of bottom-up synthetic biology, lipid membranes are the scaffold to create minimal cells and mimic reactions and processes at or across the membrane. In this context, we...
3.
Duncombe T, Dittrich P
Curr Opin Biotechnol
. 2019 Jun;
60:205-212.
PMID: 31200276
Droplet microfluidics has become a powerful analytical platform in biological research for conducting high-throughput screening in millions of discrete micro-reactors per hour. While the method facilitates faster and cheaper testing...
4.
Duncombe T, de Raad M, Bowen B, Singh A, Northen T
Anal Chem
. 2018 Aug;
90(16):9657-9661.
PMID: 30063326
Surface-assisted laser desorption ionization (SALDI) is an approach for gas-phase ion generation for mass spectrometry using laser excitation on typically conductive or semiconductive nanostructures. Here, we introduce insulator nanostructure desorption...
5.
Gao J, Sasse J, Lewald K, Zhalnina K, Cornmesser L, Duncombe T, et al.
J Vis Exp
. 2018 May;
(134).
PMID: 29708529
Beneficial plant-microbe interactions offer a sustainable biological solution with the potential to boost low-input food and bioenergy production. A better mechanistic understanding of these complex plant-microbe interactions will be crucial...
6.
Sinkala E, Sollier-Christen E, Renier C, Rosas-Canyelles E, Che J, Heirich K, et al.
Nat Commun
. 2017 Mar;
8:14622.
PMID: 28332571
Circulating tumour cells (CTCs) are rare tumour cells found in the circulatory system of certain cancer patients. The clinical and functional significance of CTCs is still under investigation. Protein profiling...
7.
Kang C, Yamauchi K, Vlassakis J, Sinkala E, Duncombe T, Herr A
Nat Protoc
. 2016 Jul;
11(8):1508-30.
PMID: 27466711
This protocol describes how to perform western blotting on individual cells to measure cell-to-cell variation in protein expression levels and protein state. Like conventional western blotting, single-cell western blotting (scWB)...
8.
Kapil M, Pan Y, Duncombe T, Herr A
Anal Chem
. 2016 Mar;
88(7):3669-76.
PMID: 26963604
High throughput, efficient, and readily adoptable analytical tools for the validation and selection of reliable antibody reagents would impact the life sciences, clinical chemistry, and clinical medicine. To directly quantify...
9.
Duncombe T, Kang C, Maity S, Ward T, Pegram M, Murthy N, et al.
Adv Mater
. 2015 Nov;
28(2):327-334.
PMID: 26567472
Pore-gradient microgel arrays enable thousands of parallel high-resolution single-cell protein electrophoresis separations for targets accross a wide molecular mass (25-289 kDa), yet within 1 mm separation distances. Dual crosslinked hydrogels...
10.
Duncombe T, Tentori A, Herr A
Nat Rev Mol Cell Biol
. 2015 Aug;
16(9):554-67.
PMID: 26296163
The underlying physical properties of microfluidic tools have led to new biological insights through the development of microsystems that can manipulate, mimic and measure biology at a resolution that has...