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Tobias Killian

Explore the profile of Tobias Killian including associated specialties, affiliations and a list of published articles. Areas
Snapshot
Articles 6
Citations 85
Followers 0
Related Specialties
Biochemistry
Science
General Medicine
Top 10 Co-Authors
Ulrich Brinkmann
Annette Buntz
Olaf Mundigl
Klaus Mayer
Heike Seul
Christine Skerka
Stefan O Reber
Gernot Langst
Susanne Ackermann
Wolfgang Schafer
Published In
Nucleic Acids Res
PLoS One
Sci Rep
Int J Mol Sci
Brain Behav Immun
Affiliations
Soon will be listed here.
Recent Articles
1.
Antibody-targeted chromatin enables effective intracellular delivery and functionality of CRISPR/Cas9 expression plasmids
Killian T, Buntz A, Herlet T, Seul H, Mundigl O, Langst G, et al.
Nucleic Acids Res . 2019 Feb; 47(10):e55. PMID: 30809660
We report a novel system for efficient and specific targeted delivery of large nucleic acids to and into cells. Plasmid DNA and core histones were assembled to chromatin by salt...
2.
Quantitative fluorescence imaging determines the absolute number of locked nucleic acid oligonucleotides needed for suppression of target gene expression
Buntz A, Killian T, Schmid D, Seul H, Brinkmann U, Ravn J, et al.
Nucleic Acids Res . 2018 Nov; 47(2):953-969. PMID: 30462278
Locked nucleic acid based antisense oligonucleotides (LNA-ASOs) can reach their intracellular RNA targets without delivery modules. Functional cellular uptake involves vesicular accumulation followed by translocation to the cytosol and nucleus....
3.
Disruption of diphthamide synthesis genes and resulting toxin resistance as a robust technology for quantifying and optimizing CRISPR/Cas9-mediated gene editing
Killian T, Dickopf S, Haas A, Kirstenpfad C, Mayer K, Brinkmann U
Sci Rep . 2017 Nov; 7(1):15480. PMID: 29133816
We have devised an effective and robust method for the characterization of gene-editing events. The efficacy of editing-mediated mono- and bi-allelic gene inactivation and integration events is quantified based on...
4.
Blocking metabotropic glutamate receptor subtype 5 relieves maladaptive chronic stress consequences
Peterlik D, Stangl C, Bauer A, Bludau A, Keller J, Grabski D, et al.
Brain Behav Immun . 2016 Aug; 59:79-92. PMID: 27524668
Etiology and pharmacotherapy of stress-related psychiatric conditions and somatoform disorders are areas of high unmet medical need. Stressors holding chronic plus psychosocial components thereby bear the highest health risk. Although...
5.
TriFabs--Trivalent IgG-Shaped Bispecific Antibody Derivatives: Design, Generation, Characterization and Application for Targeted Payload Delivery
Mayer K, Baumann A, Grote M, Seeber S, Kettenberger H, Breuer S, et al.
Int J Mol Sci . 2015 Nov; 16(11):27497-507. PMID: 26593903
TriFabs are IgG-shaped bispecific antibodies (bsAbs) composed of two regular Fab arms fused via flexible linker peptides to one asymmetric third Fab-sized binding module. This third module replaces the IgG...
6.
A novel antibody against human properdin inhibits the alternative complement system and specifically detects properdin from blood samples
Pauly D, Nagel B, Reinders J, Killian T, Wulf M, Ackermann S, et al.
PLoS One . 2014 May; 9(5):e96371. PMID: 24797388
The complement system is an essential part of the innate immune system by acting as a first line of defense which is stabilized by properdin, the sole known positive regulator...