T W J Gadella Jr
Overview
Explore the profile of T W J Gadella Jr including associated specialties, affiliations and a list of published articles.
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8
Citations
261
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Recent Articles
1.
van Unen J, Botman D, Yin T, Wu Y, Hink M, Gadella Jr T, et al.
BMC Cell Biol
. 2018 Jun;
19(1):6.
PMID: 29879899
Background: Rho guanine exchange factors (RhoGEFs) control cellular processes such as migration, adhesion and proliferation. Alternative splicing of the RhoGEF Trio produces TGAT. The RhoGEF TGAT is an oncoprotein with...
2.
Joosen L, Hink M, Gadella Jr T, Goedhart J
J Microsc
. 2014 Sep;
256(3):166-76.
PMID: 25179491
Fluorescence lifetime imaging microscopy can be used to study protein-protein interactions by Förster Resonance Energy Transfer or to perform lifetime-based multiplexing. Fixation of samples with cells producing fluorescent fusion proteins...
3.
4.
van Munster E, Goedhart J, Kremers G, Manders E, Gadella Jr T
Cytometry A
. 2007 Feb;
71(4):207-14.
PMID: 17266147
Background: Wide-field frequency-domain fluorescence lifetime imaging microscopy (FLIM) is an established technique to determine fluorescence lifetimes. Disadvantage of wide-field imaging is that measurements are compromised by out-of-focus blur. Conventional scanning...
5.
Hoebe R, Van Oven C, Gadella Jr T, Dhonukshe P, van Noorden C, Manders E
Nat Biotechnol
. 2007 Jan;
25(2):249-53.
PMID: 17237770
Fluorescence microscopy of living cells enables visualization of the dynamics and interactions of intracellular molecules. However, fluorescence live-cell imaging is limited by photobleaching and phototoxicity induced by the excitation light....
6.
van Munster E, Kremers G, Adjobo-Hermans M, Gadella Jr T
J Microsc
. 2005 Jun;
218(Pt 3):253-62.
PMID: 15958019
Fluorescence resonance energy transfer (FRET) is an extremely effective tool to detect molecular interaction at suboptical resolutions. One of the techniques for measuring FRET is acceptor photobleaching: the increase in...
7.
Vermeer J, van Munster E, Vischer N, Gadella Jr T
J Microsc
. 2004 Apr;
214(Pt 2):190-200.
PMID: 15102066
Summary Multimode fluorescence resonance energy transfer (FRET) microscopy was applied to study the plasma membrane organization using different lipidated green fluorescent protein (GFP)-fusion proteins co-expressed in cowpea protoplasts. Cyan fluorescent...
8.
phiFLIM: a new method to avoid aliasing in frequency-domain fluorescence lifetime imaging microscopy
van Munster E, Gadella Jr T
J Microsc
. 2003 Dec;
213(1):29-38.
PMID: 14678510
In conventional wide-field frequency-domain fluorescence lifetime imaging microscopy (FLIM), excitation light is intensity-modulated at megahertz frequencies. Emitted fluorescence is recorded by a CCD camera through an image intensifier, which is...