Stephane E Guillouet
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Explore the profile of Stephane E Guillouet including associated specialties, affiliations and a list of published articles.
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28
Citations
244
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Recent Articles
1.
Di Bisceglie F, Garcia Navarro J, Lombard E, Kratzer R, Kourist R, Guillouet S
Biotechnol J
. 2025 Feb;
20(2):e202400641.
PMID: 39924831
This study conceptualizes the design of a small-scale system (250 mL-1 L) for the autotrophic cultivation of hydrogen-oxidizing bacteria, such as the representative strain Cupriavidus necator. The research aimed to...
2.
Sydow A, Becker L, Lombard E, Ulber R, Guillouet S, Holtmann D
Bioengineering (Basel)
. 2023 Oct;
10(10).
PMID: 37892924
is a facultative chemolithotrophic organism that grows under both heterotrophic and autotrophic conditions. It is becoming increasingly important due to its ability to convert CO into industrially valuable chemicals. To...
3.
Boy C, Lesage J, Alfenore S, Gorret N, Guillouet S
J Biotechnol
. 2023 Mar;
366:25-34.
PMID: 36870479
Strain robustness during production of recombinant molecules is of major interest to ensure bioprocess profitability. The heterogeneity of populations has been shown in the literature as a source of instability...
4.
Boy C, Lesage J, Alfenore S, Guillouet S, Gorret N
Enzyme Microb Technol
. 2022 Sep;
161:110114.
PMID: 36070644
Phenotypic heterogeneity in bioprocesses is suspected to reduce performances, even in case of monoclonal cultures. Here, robustness of an engineered isopropanol-overproducing strain and heterogeneity of its plasmid expression level were...
5.
Boy C, Lesage J, Alfenore S, Guillouet S, Gorret N
J Biotechnol
. 2022 Jan;
345:17-29.
PMID: 34995560
Plasmid expression level heterogeneity in Cupriavidus necator was studied in response to stringent culture conditions, supposed to enhance plasmid instability, through plasmid curing strategies. Two plasmid curing strategies were compared...
6.
Boy C, Lesage J, Alfenore S, Guillouet S, Gorret N
AMB Express
. 2021 Nov;
11(1):151.
PMID: 34783891
It is of major interest to ensure stable and performant microbial bioprocesses, therefore maintaining high strain robustness is one of the major future challenges in industrial microbiology. Strain robustness can...
7.
Lesage J, Timoumi A, Cenard S, Lombard E, Lee H, Guillouet S, et al.
N Biotechnol
. 2021 May;
64:37-45.
PMID: 34058397
Yarrowia lipolytica, with a diverse array of biotechnological applications, is able to grow as ovoid yeasts or filamentous hyphae depending on environmental conditions. This study has explored the relationship between...
8.
Garrigues L, Do T, Bideaux C, Guillouet S, Meynial-Salles I
Biotechnol Adv
. 2021 May;
54:107781.
PMID: 34029623
Tetanus vaccination is of major importance for public health in most countries in the world. The World Health Organization indicated that 15,000 tetanus cases were reported in 2018 (Organization, World...
9.
Boy C, Lesage J, Alfenore S, Gorret N, Guillouet S
Appl Microbiol Biotechnol
. 2020 May;
104(13):5899-5914.
PMID: 32358761
A methodology for plasmid expression level monitoring of eGFP expression suitable for dynamic processes was assessed during fermentation. This technique was based on the expression of a fluorescent biosensor (eGFP)...
10.
Garrigues L, Maignien L, Lombard E, Singh J, Guillouet S
N Biotechnol
. 2019 Nov;
56:16-20.
PMID: 31731039
A bioreactor was designed to provide high gas mass transfer to reach cell and product titres in the g L level from CO for realistic, laboratory scale, engineered autotrophic strain...