Stephan Nickell
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Explore the profile of Stephan Nickell including associated specialties, affiliations and a list of published articles.
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25
Citations
1353
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Recent Articles
1.
Nagy I, Knispel R, Kofler C, Orsini M, Boicu M, Varga S, et al.
FEMS Microbiol Lett
. 2016 Jul;
363(18).
PMID: 27405311
Cytosolic, globular droplets with an average diameter of 50 nm were observed in vitrified Thermoplasma acidophilum cells by means of cryo-electron tomography. These droplets were isolated by column chromatography and...
2.
Eibauer M, Hoffmann C, Plitzko J, Baumeister W, Nickell S, Engelhardt H
J Struct Biol
. 2012 Sep;
180(3):488-96.
PMID: 23000705
Cryo-electron tomography in combination with subtomogram averaging allows to investigate the structure of protein assemblies in their natural environment in a close to live state. To make full use of...
3.
Beck F, Unverdorben P, Bohn S, Schweitzer A, Pfeifer G, Sakata E, et al.
Proc Natl Acad Sci U S A
. 2012 Aug;
109(37):14870-5.
PMID: 22927375
The 26S proteasome operates at the executive end of the ubiquitin-proteasome pathway. Here, we present a cryo-EM structure of the Saccharomyces cerevisiae 26S proteasome at a resolution of 7.4 Å...
4.
Knispel R, Kofler C, Boicu M, Baumeister W, Nickell S
Nat Methods
. 2012 Jan;
9(2):182-4.
PMID: 22231640
We report a simple and generic method for the direct transfer of protein complexes separated by native gel electrophoresis to electron microscopy grids. After transfer, sufficient material remains in the...
5.
Sakata E, Bohn S, Mihalache O, Kiss P, Beck F, Nagy I, et al.
Proc Natl Acad Sci U S A
. 2012 Jan;
109(5):1479-84.
PMID: 22215586
Two canonical subunits of the 26S proteasome, Rpn10 and Rpn13, function as ubiquitin (Ub) receptors. The mutual arrangement of these subunits--and all other non-ATPase subunits--in the regulatory particle is unknown....
6.
Pathare G, Nagy I, Bohn S, Unverdorben P, Hubert A, Korner R, et al.
Proc Natl Acad Sci U S A
. 2011 Dec;
109(1):149-54.
PMID: 22187461
Proteasomes execute the degradation of most cellular proteins. Although the 20S core particle (CP) has been studied in great detail, the structure of the 19S regulatory particle (RP), which prepares...
7.
Korinek A, Beck F, Baumeister W, Nickell S, Plitzko J
J Struct Biol
. 2011 Jun;
175(3):394-405.
PMID: 21704708
Automated data acquisition expedites structural studies by electron microscopy and it allows to collect data sets of unprecedented size and consistent quality. In electron tomography it greatly facilitates the systematic...
8.
Bohn S, Beck F, Sakata E, Walzthoeni T, Beck M, Aebersold R, et al.
Proc Natl Acad Sci U S A
. 2010 Nov;
107(49):20992-7.
PMID: 21098295
The structure of the 26S proteasome from Schizosaccharomyces pombe has been determined to a resolution of 9.1 Å by cryoelectron microscopy and single particle analysis. In addition, chemical cross-linking in...
9.
Stolken M, Beck F, Haller T, Hegerl R, Gutsche I, Carazo J, et al.
J Struct Biol
. 2010 Aug;
173(1):77-85.
PMID: 20719249
Classification and averaging of sub-tomograms can improve the fidelity and resolution of structures obtained by electron tomography. Here we present a three-dimensional (3D) maximum likelihood algorithm--MLTOMO--which is characterized by integrating...
10.
Sun N, Pan C, Nickell S, Mann M, Baumeister W, Nagy I
J Proteome Res
. 2010 Jul;
9(9):4839-50.
PMID: 20669988
A comparative proteome and transcriptome analysis of Thermoplasma acidophilum cultured under aerobic and anaerobic conditions has been performed. One-thousand twenty-five proteins were identified covering 88% of the cytosolic proteome. Using...