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Shusei Obata

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Articles 11
Citations 148
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Recent Articles
1.
Sui Y, Maruyama S, Okada N, Ito M, Muramatsu M, Obata S, et al.
Biosci Biotechnol Biochem . 2023 May; 87(8):925-932. PMID: 37156521
PsADH, an alcohol dehydrogenase originating in Pantoea sp. was characterized and found to convert a broad variety of fatty alcohols into their corresponding aldehydes, the substrates of alkane biosynthesis. By...
2.
Sui Y, Kishino S, Maruyama S, Ito M, Muramatsu M, Obata S, et al.
Appl Environ Microbiol . 2022 Nov; 88(23):e0126422. PMID: 36416567
Alkanes produced by microorganisms are expected to be an alternative to fossil fuels as an energy source. Microbial synthesis of alkanes involves the formation of fatty aldehydes via fatty acyl...
3.
Suzuki T, Sakamoto T, Sugiyama M, Ishida N, Kambe H, Obata S, et al.
J Biosci Bioeng . 2013 Jan; 115(5):467-74. PMID: 23290995
To create strains that have high productivity of lactic acid without neutralization, a genome-wide screening for strains showing hyper-resistance to 6% l-lactic acid (pH 2.6) was performed using the gene...
4.
Ohto C, Muramatsu M, Obata S, Sakuradani E, Shimizu S
Appl Microbiol Biotechnol . 2010 Apr; 87(4):1327-34. PMID: 20393702
An acyclic diterpene alcohol, (E,E,E)-geranylgeraniol (GGOH), is one of the important compounds used as perfume and pharmacological agents. A deficiency of squalene (SQ) synthase activity allows yeasts to accumulate an...
5.
Tokuhiro K, Muramatsu M, Ohto C, Kawaguchi T, Obata S, Muramoto N, et al.
Appl Environ Microbiol . 2009 Jul; 75(17):5536-43. PMID: 19592534
(E, E, E)-Geranylgeraniol (GGOH) is a valuable starting material for perfumes and pharmaceutical products. In the yeast Saccharomyces cerevisiae, GGOH is synthesized from the end products of the mevalonate pathway...
6.
Muramatsu M, Ohto C, Obata S, Sakuradani E, Shimizu S
J Biosci Bioeng . 2009 Jul; 108(1):52-5. PMID: 19577192
External environments affect prenyl alcohol production by squalene synthetase-deficient mutant Saccharomyces cerevisiae ATCC 64031. Cultivation of the yeast in medium with an initial pH ranging from 7.0 to 8.0 increased...
7.
Ohto C, Muramatsu M, Obata S, Sakuradani E, Shimizu S
Biosci Biotechnol Biochem . 2009 Jan; 73(1):186-8. PMID: 19129660
Isopentenyl diphosphate isomerase (idi) and farnesyl diphosphate synthase (ispA) genes were overexpressed in Escherichia coli. The resulting transformant showed 6.8-fold higher production of farnesol (389 microg/l). In a similar manner,...
8.
Ohto C, Muramatsu M, Obata S, Sakuradani E, Shimizu S
Appl Microbiol Biotechnol . 2008 Dec; 82(5):837-45. PMID: 19083230
To develop microbial production method for prenyl alcohols (e.g., (E,E)-farnesol (FOH), (E)-nerolidol (NOH), and (E,E,E)-geranylgeraniol (GGOH)), the genes encoding enzymes in the mevalonate and prenyl diphosphate pathways were overexpressed in...
9.
Taguchi S, Yamada M, Matsumoto K, Tajima K, Satoh Y, Munekata M, et al.
Proc Natl Acad Sci U S A . 2008 Nov; 105(45):17323-7. PMID: 18978031
Polylactate (PLA) is synthesized as a representative bio-based polyester by the chemo-bio process on the basis of metal catalyst-mediated chemical polymerization of lactate (LA) supplied by microbial fermentation. To establish...
10.
Muramatsu M, Ohto C, Obata S, Sakuradani E, Shimizu S
J Biosci Bioeng . 2008 Oct; 106(3):263-7. PMID: 18930003
The object of this research was improvement of prenyl alcohol production with squalene synthase-deficient mutant Saccharomyces cerevisiae ATCC 64031. On screening of many kinds of additives, we found that oils...