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Shaun M McLoughlin

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Articles 13
Citations 346
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Recent Articles
1.
Joignant A, Pu F, McLoughlin S, Sawicki J, Radosevich A, Ma R, et al.
SLAS Technol . 2025 Mar; :100272. PMID: 40088972
The adoption of mass spectrometry for high-throughput screening in drug discovery has become increasingly prevalent and has enabled label-free screening against diverse targets. Cellular assays for phenotypic screening, however, are...
2.
Comess K, McLoughlin S, Oyer J, Richardson P, Stockmann H, Vasudevan A, et al.
J Med Chem . 2018 May; 61(19):8504-8535. PMID: 29718665
Small-molecule (SM) leads in the early drug discovery pipeline are progressed primarily based on potency against the intended target(s) and selectivity against a very narrow slice of the proteome. So,...
3.
Wilsbacher J, Cheng M, Cheng D, Trammell S, Shi Y, Guo J, et al.
Mol Cancer Ther . 2017 May; 16(7):1236-1245. PMID: 28468779
Cancer cells are highly reliant on NAD-dependent processes, including glucose metabolism, calcium signaling, DNA repair, and regulation of gene expression. Nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting enzyme for NAD salvage from...
4.
Tang H, Duggan S, Richardson P, Marin V, Warder S, McLoughlin S
J Biomol Screen . 2015 Dec; 21(2):201-11. PMID: 26676096
The pharmaceutical industry has been continually challenged by dwindling target diversity. To obviate this trend, phenotypic screens have been adopted, complementing target-centric screening approaches. Phenotypic screens identify drug leads using...
5.
Zhang H, McLoughlin S, Frausto S, Tang H, Emmett M, Marshall A
Anal Chem . 2010 Jan; 82(4):1450-4. PMID: 20099838
Proteolyzed peptides provide the basis for mass-analyzed hydrogen/deuterium exchange (HDX) for mapping solvent access to various segments of solution-phase proteins. Aspergillus saitoi protease type XIII and porcine pepsin can generate...
6.
Warder S, Tucker L, McLoughlin S, Strelitzer T, Meuth J, Zhang Q, et al.
J Proteome Res . 2008 Oct; 7(11):4807-20. PMID: 18828628
The catalytic activity of methionine aminopeptidase-2 (MetAP2) has been pharmacologically linked to cell growth, angiogenesis, and tumor progression, making this an attractive target for cancer therapy. An assay for monitoring...
7.
McLoughlin S, Kelleher N
J Am Chem Soc . 2005 Oct; 127(43):14984-5. PMID: 16248611
For the uninterrupted observation of natural product bioassembly on nonribosomal peptide synthetases, Quadrupole Fourier Transform Mass Spectrometry (Q-FTMS) was utilized to directly interrogate peptides harboring covalently modified residues in yersiniabactin...
8.
McLoughlin S, Mazur M, Miller L, Yin J, Liu F, Walsh C, et al.
Biochemistry . 2005 Oct; 44(43):14159-69. PMID: 16245932
For the direct interrogation of peptides harboring covalently modified serines in nonribosomal peptide synthetases, streamlined methodologies described here employ proteolysis and reporter-coenzyme A analogues of four types. The chromophoric and...
9.
Yin J, Straight P, McLoughlin S, Zhou Z, Lin A, Golan D, et al.
Proc Natl Acad Sci U S A . 2005 Oct; 102(44):15815-20. PMID: 16236721
An 11-residue peptide with the sequence DSLEFIASKLA was identified from a genomic library of Bacillus subtilis by phage display as an efficient substrate for Sfp phosphopantetheinyl transferase-catalyzed protein labeling by...
10.
Miller L, Mazur M, McLoughlin S, Kelleher N
Protein Sci . 2005 Oct; 14(10):2702-12. PMID: 16195555
For determination of multiple covalent intermediates bound to the ultra-large enzymes responsible for biosynthesis via nonribosomal peptide synthesis, mass spectrometry (MS) is a promising method to provide new mechanistic insight....