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S N NAYFEH

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Citations 190
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11.
McQuade R, THOMAS Jr C, NAYFEH S
Biochem Biophys Res Commun . 1986 May; 137(1):61-8. PMID: 3013196
Following cross-linking with disuccinimidyl suberate and analysis by SDS-PAGE and autoradiography, both the high- and low-affinity TSH binding components exhibited two similar 125I-TSH-labeled bands, with Mr values of 80,000 and...
12.
McQuade R, THOMAS Jr C, NAYFEH S
Arch Biochem Biophys . 1986 Apr; 246(1):52-62. PMID: 3008661
The subunit composition of the thyrotropin (TSH) receptor has been characterized using the bifunctional crosslinking agent, disuccinimidyl suberate (DSS), to covalently link [125I]TSH to its receptor. Purified thyroid membranes were...
13.
Gennick S, THOMAS Jr C, NAYFEH S
Biochem Biophys Res Commun . 1986 Feb; 135(1):208-14. PMID: 3006679
125I-TSH binding to porcine thyroid and guinea pig fat resulted in curvilinear Scatchard plots with similar dissociation constants for the high and low affinity binding components. Antibodies from the sera...
14.
Berman M, THOMAS Jr C, NAYFEH S
J Cyclic Nucleotide Protein Phosphor Res . 1986 Jan; 11(2):99-111. PMID: 3016048
Addition of N6-(L-2-phenylisopropyl)-adenosine (PIA) to cultured FRTL-5 rat thyroid cells led to a concentration-dependent inhibition of TSH-stimulated cAMP formation. Half-maximal inhibition was attained with approximately 0.5 nM PIA. Forskolin and...
15.
Berman M, THOMAS Jr C, Manjunath P, Sairam M, NAYFEH S
Biochem Biophys Res Commun . 1985 Dec; 133(2):680-7. PMID: 3002351
The relative binding affinity of deglycosylated human TSH was 6-fold higher than that of native TSH. Although deglycosylated human TSH significantly stimulated adenylate cyclase, it was less effective than the...
16.
THOMAS Jr C, Combest W, McQuade R, Jordan H, Reddick R, NAYFEH S
World J Surg . 1984 Aug; 8(4):445-51. PMID: 6091346
No abstract available.
17.
Saltiel A, THOMAS Jr C, NAYFEH S
Mol Cell Endocrinol . 1982 Nov; 28(3):299-312. PMID: 7152094
Evaluation of TSH binding to plasma membranes of porcine thyroid revealed unique sensitivity to pH and temperature. Analysis of apparent equilibrium binding yielded a linear Scatchard plot at the optimal...
18.
McQuade R, THOMAS Jr C, NAYFEH S
Mol Cell Endocrinol . 1982 Nov; 28(3):275-87. PMID: 6295838
Following radioiodination by the lactoperoxidase method and subsequent purification on Sephadex G100, it was found that [125I]TSH exhibited varying degrees of binding activities to the thyrotropin receptor. In order to...
19.
Drummond R, McQuade R, Grunwald R, THOMAS Jr C, NAYFEH S
Proc Natl Acad Sci U S A . 1982 Apr; 79(7):2202-6. PMID: 6285372
Two distinct thyrotropin (TSH) binding species have been separated from solubilized porcine thyroid membranes. Membranes was solubilized with 1% Triton X-100, and the supernatant was recovered by centrifugation at 105,000...
20.
Saltiel A, THOMAS Jr C, NAYFEH S
Mol Cell Endocrinol . 1981 Nov; 24(2):219-31. PMID: 6271612
The kinetics of the dissociation of thyrotropin (TSH) from human thyroid plasma membranes were studied in an attempt to further understand the molecular dynamics of the TSH--receptor interaction. Dissociation of...