Robert C Leif
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Explore the profile of Robert C Leif including associated specialties, affiliations and a list of published articles.
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13
Citations
108
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Recent Articles
1.
Wang Y, Sayyadi N, Zheng X, Woods T, Leif R, Shi B, et al.
Lab Chip
. 2020 Jan;
20(3):655-664.
PMID: 31934716
Time-resolved luminescence detection using long-lived probes with lifetimes in the microsecond region have shown great potential in ultrasensitive and multiplexed bioanalysis. In flow cytometry, however, the long lifetime poses a...
2.
Zheng X, Lu Y, Zhao J, Zhang Y, Ren W, Liu D, et al.
Anal Chem
. 2015 Dec;
88(2):1312-9.
PMID: 26669618
Compared with routine microscopy imaging of a few analytes at a time, rapid scanning through the whole sample area of a microscope slide to locate every single target object offers...
3.
Lu Y, Lu J, Zhao J, Cusido J, Raymo F, Yuan J, et al.
Nat Commun
. 2014 May;
5:3741.
PMID: 24796249
Significant multiplexing capacity of optical time-domain coding has been recently demonstrated by tuning luminescence lifetimes of the upconversion nanoparticles called 'τ-Dots'. It provides a large dynamic range of lifetimes from...
4.
Jin D, Lu Y, Leif R, Yang S, Rajendran M, Miller L
Curr Protoc Cytom
. 2014 Feb;
67:2.22.1-2.22.36.
PMID: 24510771
The sensitivity of filter-based fluorescence microscopy techniques is limited by autofluorescence background. Time-gated detection is a practical way to suppress autofluorescence, enabling higher contrast and improved sensitivity. In the past...
5.
Lu Y, Jin D, Leif R, Deng W, Piper J, Yuan J, et al.
Cytometry A
. 2011 Apr;
79(5):349-55.
PMID: 21462305
Many microorganisms have a very low threshold (<10 cells) to trigger infectious diseases, and, in these cases, it is important to determine the absolute cell count in a low-cost and...
6.
Leif R
J Biophotonics
. 2009 Jun;
2(8-9):482-93.
PMID: 19504518
The integration of cytomics research and healthcare informatics will facilitate technology transfer and reduce medical costs. The CytometryML prototype of the Advanced Cytometry Standard (ACS) has the benefits of including...
7.
Jin D, Piper J, Leif R, Yang S, Ferrari B, Yuan J, et al.
J Biomed Opt
. 2009 May;
14(2):024023.
PMID: 19405753
A fundamental problem for rare-event cell analysis is auto-fluorescence from nontarget particles and cells. Time-gated flow cytometry is based on the temporal-domain discrimination of long-lifetime (>1 micros) luminescence-stained cells and...
8.
Leif R, Yang S, Jin D, Piper J, Vallarino L, Williams J, et al.
J Biomed Opt
. 2009 May;
14(2):024022.
PMID: 19405752
The reliability of lanthanide luminescence measurements, by both flow cytometry and digital microscopy, would be enhanced by the availability of narrowband emitting, UV excited lanthanide calibration beads. 0.5-, 3-, and...
9.
Spidlen J, Leif R, Moore W, Roederer M, Brinkman R
Cytometry A
. 2008 Sep;
73A(12):1151-7.
PMID: 18773465
The lack of software interoperability with respect to gating due to lack of a standardized mechanism for data exchange has traditionally been a bottleneck, preventing reproducibility of flow cytometry (FCM)...
10.
Leif R, Vallarino L, Becker M, Yang S
Cytometry A
. 2006 Sep;
69(8):940-6.
PMID: 16969811
Background: Luminescent lanthanide complexes produce emissions with the narrowest-known width at half maximum; however, their significant use in cytometry required an increase in luminescence intensity. The companion review, Leif et...