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Raymond M D Verhaert

Explore the profile of Raymond M D Verhaert including associated specialties, affiliations and a list of published articles. Areas
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Articles 7
Citations 58
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Recent Articles
1.
Bonzom C, Huttner S, Mirgorodskaya E, Chong S, Uthoff S, Steinbuchel A, et al.
AMB Express . 2019 Aug; 9(1):126. PMID: 31407106
Heterologous protein production is widely used in industrial biotechnology. However, using non-native production hosts can lead to enzymes with altered post-translational modifications, such as glycosylation. We have investigated how production...
2.
Gidijala L, Uthoff S, van Kampen S, Steinbuchel A, Verhaert R
Microb Cell Fact . 2018 Jul; 17(1):112. PMID: 30005638
Background: The yeast Komagataella phaffii, better known as Pichia pastoris, is a commonly used host for recombinant protein production. Here expression vectors are reported that address the different steps of...
3.
Ramos J, Barends S, Verhaert R, de Graaff L
Microb Cell Fact . 2011 Oct; 10:78. PMID: 21981827
Background: Many filamentous fungal genomes contain complex groups of multicopper oxidase (MCO) coding genes that makes them a good source for new laccases with potential biotechnological interest. A bioinformatics analysis...
4.
Droge M, Ruggeberg C, van der Sloot A, Schimmel J, Dijkstra D, Verhaert R, et al.
J Biotechnol . 2003 Jan; 101(1):19-28. PMID: 12523966
Phage display can be used as a protein engineering tool to select proteins with desirable binding properties from a library of randomly constructed mutants. Here, we describe the development of...
5.
Sio C, Riemens A, van der Laan J, Verhaert R, Quax W
Eur J Biochem . 2002 Sep; 269(18):4495-504. PMID: 12230561
Semi-synthetic cephalosporin antibiotics belong to the top 10 of most sold drugs, and are produced from 7-aminodesacetoxycephalosporanic acid (7-ADCA). Recently new routes have been developed which allow for the production...
6.
Verhaert R, Beekwilder J, Olsthoorn R, van Duin J, Quax W
J Biotechnol . 2002 Jul; 96(1):103-18. PMID: 12142147
Directed evolution of secreted industrial enzymes is hampered by the lack of powerful selection techniques. We have explored surface display to select for enzyme variants with improved binding performance on...
7.
van Meerten D, Olsthoorn R, van Duin J, Verhaert R
J Gen Virol . 2001 Jun; 82(Pt 7):1797-1805. PMID: 11413393
The potential of the RNA phage MS2 to accommodate extra amino acids in its major coat protein has been examined. Accordingly, a pentapeptide was encoded in the genome as an...