R W Horobin
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Explore the profile of R W Horobin including associated specialties, affiliations and a list of published articles.
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121
Citations
576
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Recent Articles
1.
Stockert J, Durantini E, Gonzalez Lopez E, Durantini J, Villanueva A, Horobin R
Biotech Histochem
. 2022 Jul;
97(7):473-479.
PMID: 35852233
The study of labeling selectivity and mechanisms of fluorescent organelle probes in living cells is of continuing interest in biomedical sciences. The tetracationic phthalocyanine-like ZnTM2,3PyPz photosensitizing dye induces a selective...
2.
Ali S, Dapson R, Horobin R, Kiernan J, Kazlauciunas A
Biotech Histochem
. 2022 Jan;
97(1):11-20.
PMID: 35026959
We investigated physicochemical characteristics of dye lots sold as "alcian blue" using the Biological Stain Commission (BSC) precipitation test, differential scanning calorimetry, high performance liquid chromatography, thin layer chromatography and...
3.
Fagan C, Dapson R, Horobin R, Kiernan J
Biotech Histochem
. 2020 Feb;
95(5):333-340.
PMID: 32022609
Alcian blue dyes are copper phthalocyanines with a variety of cationic side chains; they are useful for staining carbohydrate polyanions while avoiding staining of nucleic acids. The properties of the...
4.
Hope-Roberts M, Horobin R
Biotech Histochem
. 2017 May;
92(5):315-323.
PMID: 28506128
Curcumin has been widely used to color textiles but, unlike other natural dyes such as hematoxylin or saffron, it rarely has been discussed as a biological stain. Aspects of the...
5.
Horobin R, Stockert J, Rashid-Doubell F
Biotech Histochem
. 2015 Mar;
90(4):241-54.
PMID: 25801297
We discuss a variety of biological targets including generic biomembranes and the membranes of the endoplasmic reticulum, endosomes/lysosomes, Golgi body, mitochondria (outer and inner membranes) and the plasma membrane of...
6.
Horobin R, Stockert J, Rashid-Doubell F
Biotech Histochem
. 2015 Mar;
90(4):255-63.
PMID: 25801295
We provide detailed protocols for applying the QSAR decision-rule models described in Part 2 of this paper. These procedures permit prediction of the intracellular localization of fluorescent probes or of...
7.
Lyon H, Horobin R
Biotech Histochem
. 2014 Mar;
89(3):232-9.
PMID: 24646106
In the 15(th) issue of News from the Biological Stain Commission (BSC), under the heading of Regulatory affairs, the Biological Stain Commission's International Affairs Committee presents information from the plenary...
8.
Horobin R, Rashid-Doubell F
Biotech Histochem
. 2013 Jun;
88(8):461-76.
PMID: 23758208
We describe the practical issues and the methodological procedures that must be carried out to construct and use QSAR models for predicting localization of probes in single cells. We address...
9.
Horobin R, Rashid-Doubell F, Pediani J, Milligan G
Biotech Histochem
. 2013 Jun;
88(8):440-60.
PMID: 23758207
Small molecule fluorochromes (synonyms: biosensors, chemosensors, fluorescent probes, vital stains) are widely used to investigate the structure, composition, physicochemical properties and biological functions of living cells, tissues and organisms. Selective...
10.
Thompson K, Dockery P, Horobin R
Biotech Histochem
. 2012 Sep;
87(7):468-83.
PMID: 22984940
Stimulated by difficulties experienced when using fluo-3 AM, we developed a general mechanistic model to aid understanding and practical application of calcium probes applied as acetoxymethyl (AM) esters. Several practical...